piRNA silencing contributes to interspecies hybrid sterility and reproductive isolation in Drosophila melanogaster

Nucleic Acids Research
Alexei A KotovLudmila V Olenina

Abstract

The piRNA pathway is an adaptive mechanism that maintains genome stability by repression of selfish genomic elements. In the male germline of Drosophila melanogaster repression of Stellate genes by piRNAs generated from Supressor of Stellate (Su(Ste)) locus is required for male fertility, but both Su(Ste) piRNAs and their targets are absent in other Drosophila species. We found that D. melanogaster genome contains multiple X-linked non-coding genomic repeats that have sequence similarity to the protein-coding host gene vasa. In the male germline, these vasa-related AT-chX repeats produce abundant piRNAs that are antisense to vasa; however, vasa mRNA escapes silencing due to imperfect complementarity to AT-chX piRNAs. Unexpectedly, we discovered AT-chX piRNAs target vasa of Drosophila mauritiana in the testes of interspecies hybrids. In the majority of hybrid flies, the testes were strongly reduced in size and germline content. A minority of hybrids maintained wild-type array of premeiotic germ cells in the testes, but in them harmful Stellate genes were derepressed due to the absence of Su(Ste) piRNAs, and meiotic failures were observed. Thus, the piRNA pathway contributes to reproductive isolation between D. melanogaster and c...Continue Reading

References

Sep 2, 2003·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Oct 28, 2003·Nature Cell Biology·Michelle A LiDorothea Godt
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Jul 16, 2004·Molecular and Cellular Biology·Alexei A AravinVladimir A Gvozdev
Aug 4, 2004·Molecular Phylogenetics and Evolution·Matthew D Dean, J William O Ballard
Jul 1, 2006·Science·Vasily V VaginPhillip D Zamore
Apr 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ai Khim Lim, Toshie Kai
Nov 9, 2007·PLoS Biology·Patrick M Ferree, Daniel A Barbash
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jan 30, 2010·Bioinformatics·Aaron R Quinlan, Ira M Hall
Sep 14, 2011·Annual Review of Genetics·Shamoni Maheshwari, Daniel A Barbash
Nov 29, 2012·PLoS Biology·Erin S KelleherDaniel A Barbash
Jan 30, 2013·Analytical Biochemistry·Mikhail V KibanovLudmila V Olenina
Apr 5, 2013·Evolution; International Journal of Organic Evolution·Alfred SimkinJeffrey D Jensen
Jun 13, 2014·Methods in Molecular Biology·Christophe Antoniewski
May 23, 2015·Genes & Development·Wee Siong Sho GohGregory J Hannon
Jan 27, 2016·Trends in Biochemical Sciences·Benjamin Czech, Gregory J Hannon
Mar 8, 2016·Nature·Anastassios VourekasZissimos Mourelatos
Jun 14, 2016·Molecular Cell·Yung-Chia Ariel ChenAlexei A Aravin
Jun 21, 2016·European Journal of Cell Biology·Sergei S RyazanskyLudmila V Olenina
Aug 6, 2017·Results and Problems in Cell Differentiation·Mehrnoush Dehghani, Paul Lasko
Sep 19, 2017·Developmental Cell·Swapnil S ParhadWilliam E Theurkauf

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Citations

May 21, 2020·Frontiers in Cell and Developmental Biology·Aleksei S ShatskikhLudmila V Olenina
Nov 29, 2020·Molecular Biology and Evolution·Florian SchwarzRobert Kofler
Sep 22, 2020·Trends in Genetics : TIG·Anne Ramat, Martine Simonelig
Jan 23, 2021·Nature Communications·Christopher M WardMarc F Schetelig
Feb 16, 2021·Frontiers in Genetics·Vladimir E AdashevLudmila V Olenina
Jun 24, 2021·The Plant Journal : for Cell and Molecular Biology·Suresh PokhrelBlake C Meyers
Sep 3, 2021·PLoS Genetics·Anahi Molla Herman, Emilie Brasset
Dec 19, 2021·Molecular Biology and Evolution·Iskander SaidDaniel A Barbash

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Datasets Mentioned

BETA
GM15916
GG25145
GE20836
GJ11891
GF14338
GSE120802

Methods Mentioned

BETA
RNA-seq
electrophoresis
PCR
confocal microscopy

Software Mentioned

py script
UCSC Genome Browser
cutadapt
signature
BLAST
chX
rpm
AT
Imaris®5
BitplaneAG

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