PirVP genes causing AHPND identified in a new Vibrio species (Vibrio punensis) within the commensal Orientalis clade

Scientific Reports
Leda RestrepoAlejandro Reyes Muñoz

Abstract

Acute hepatopancreatic necrosis disease (AHPND) has extended rapidly, causing alarming shrimp mortalities. Initially, the only known causative agent was Vibrio parahaemolyticus carrying a plasmid coding for the mortal toxins PirVP. Recently, it has been found that the plasmid and hence the disease, could be transferred among members of the Harveyi clade. The current study performs a genomic characterization of an isolate capable of developing AHPND in shrimp. Mortality studies and molecular and histopathological analyses showed the infection capacity of the strain. Multilocus sequence analysis placed the bacteria as a member of the Orientalis clade, well known for containing commensal and even probiotic bacteria used in the shrimp industry. Further whole genome comparative analyses, including Vibrio species from the Orientalis clade, and phylogenomic metrics (TETRA, ANI and DDH) showed that the isolate belongs to a previously unidentified species, now named Vibrio punensis sp. nov. strain BA55. Our findings show that the gene transfer capacity of Vibrio species goes beyond the clade classification, demonstrating a new pathogenic capacity to a previously known commensal clade. The presence of these genes in a different Vibrio cl...Continue Reading

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Citations

May 19, 2020·Marine Life Science & Technology·Xiao-Hua ZhangBrian Austin
Aug 18, 2020·Emerging Microbes & Infections·Phuong Thi Ngoc TranPeter Bossier
Apr 26, 2019·Diseases of Aquatic Organisms·Arun K DharYa-Sheng Juan
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Nov 30, 2019·Diseases of Aquatic Organisms·Gerardo de Carrillo-MéndezCésar Salvador Cardona-Félix
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Jul 3, 2020·Reviews in Aquaculture·Kelly ThornberCharles R Tyler
Apr 25, 2020·Journal of Invertebrate Pathology·Corey C HoltMark van der Giezen
Feb 4, 2021·Environmental Microbiology Reports·Mengzhao XuQichao Tu
Feb 18, 2021·Journal of Invertebrate Pathology·Quinn M PowersArun K Dhar
Aug 7, 2021·Journal of Animal Physiology and Animal Nutrition·Arvind D DiwanArchana N Panche

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Datasets Mentioned

BETA
KM067908
KX268305
PRJNA412371

Methods Mentioned

BETA
PCR
RAPD

Software Mentioned

GLIMMER
RNAmmer
BLAST
IslandPick
IslandViewer
MEGA
HMM
SIGI
SplitsTree4
R

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