Piscidin-1, an antimicrobial peptide from fish (hybrid striped bass morone saxatilis x M. chrysops), induces apoptotic and necrotic activity in HT1080 cells

Zoological Science
Heng-Ju LinCheng-Hui Lin

Abstract

Piscidin-1, a 22-residue cationic peptide isolated from mast cells of a hybrid striped bass, has potent antimicrobial activities against both gram-positive and -negative bacteria. To date, there is no report of its antitumor activity on any tumor cell lines. In this study, we examined the antitumor activity of a synthetic piscidin-1 peptide against several human cancer cell lines using an MTS assay and soft-agar colony-formation assay. We found that a low dose of piscidin induces both apoptosis and necrosis in HT1080 cells, as shown by annexin-V/propidium iodide and acridine orange/ethidium bromide staining, and triggers a necrotic cell death pathway in a short period with high-dose treatment. The destruction of cell membranes by piscidin-1 was demonstrated by transmission electron microscopy. Furthermore, piscidin-1 also inhibits the migration of HT1080 cells in a dose-dependent manner. This study provides the first evidence of the anticancer activity of the antimicrobial peptide, piscidin-1, with potential implications for the treatment of cancer.

References

Nov 20, 2001·Nature·U Silphaduang, E J Noga
Jun 7, 2003·Drug News & Perspectives·Edward J Noga, Umaporn Silphaduang
Dec 28, 2006·Diseases of Aquatic Organisms·U SilphaduangE J Noga
Jan 27, 2007·Biochemistry·Sylvie CampagnaAndré Aumelas
May 1, 2008·Biochemical and Biophysical Research Communications·Woo Sang SungDong Gun Lee
May 13, 2008·Journal of Fish Diseases·A ColorniE J Noga

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Citations

Mar 7, 2014·Pharmaceuticals·Jorge A Masso-Silva, Gill Diamond
Feb 10, 2016·Molecular Membrane Biology·Simon G Patching
Jan 28, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Ai-Ling LiangZhi-Guang Tu
Sep 23, 2016·Biophysical Journal·B Scott PerrinRichard W Pastor
Apr 21, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Hannah CetukSergei Sukharev
Mar 6, 2018·Journal of the American Chemical Society·Nathaniel H ParkJames L Hedrick

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Methods Mentioned

BETA
nuclear magnetic resonance
PCR
light microscopy
transmission electron microscopy

Software Mentioned

SPOT
Gallios
SPSS

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