Pit-1 gene expression in human pituitary adenomas using the reverse transcription polymerase chain reaction method

Clinical Endocrinology
S YamadaM Usui

Abstract

Previous studies of Pit-1 expression in human pituitary tumours have produced conflicting results. We have studied expression of Pit-1 mRNA in human pituitary adenomas, as well as in normal human and rat pituitaries, and results were compared with clinical, histological, and immunohistochemical features. In addition, expression of GH, PRL, and TSH-beta mRNA was also studied and compared with Pit-1 gene expression. The adenomas consisted of 13 GH cell adenomas, 7 PRL cell adenomas, 3 TSH cell adenomas, 4 ACTH cell adenomas, and 10 clinically non-functioning adenomas. Expression of the Pit-1, its isoforms, and each hormone, was studied using the reverse transcription polymerase chain reaction. Pit-1 mRNA was expressed not only in normal human and rat pituitaries, but also in all GH, PRL and TSH cell adenomas. There was no correlation between Pit-1 transcripts and biological behaviour or histological findings in these three types of adenoma, suggesting that Pit-1 is generally required for the determination of cell phenotype but is insufficient for the regulation of hormonal activity and tumour growth in these pituitary adenomas. In addition, Pit-1 was also expressed in some ACTH cell (2/4) and non-functioning adenomas (7/10). Alth...Continue Reading

Citations

Feb 18, 2004·The Journal of Reproduction and Development·Vishwajit S Chowdhury, Yukinori Yoshimura
May 2, 2012·Journal of Neurosurgery. Pediatrics·Mitsuo Yamaguchi-OkadaShozo Yamada
May 9, 2000·Journal of Endocrinological Investigation·M BarausseR Cozzi
Jul 17, 1998·Clinical Endocrinology·S IkuyamaH Nawata
Apr 16, 1999·Endocrine Reviews·P L Dahia, A B Grossman
Jun 1, 1997·The Journal of Clinical Endocrinology and Metabolism·N J GittoesJ A Franklyn
Jan 7, 2015·Endocrinology and Metabolism·Sang Ouk ChinSung Woon Kim

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