PMID: 11927395Apr 3, 2002Paper

Pit-1/GHF-1 and GH expression in the MCF-7 human breast adenocarcinoma cell line

The Journal of Endocrinology
C Gil-PuigR Perez-Fernandez

Abstract

GH expression in mammary tumors has been related to the increase and spreading of cell proliferation. Using the MCF-7 human breast adenocarcinoma cell line, it has been demonstrated that autocrine GH-stimulated mammary carcinoma cell proliferation decreased the apoptosis rate and enhanced cell spreading. Surprisingly, no data are available about the presence of Pit-1 (the main pituitary regulator of GH) or GH expression in this cell line. Using RT-PCR, Western blot and immunohistochemistry, we have demonstrated the presence of both mRNA coding Pit-1 and GH as well as Pit-1 and GH protein in the MCF-7 cell line. These data could imply that Pit-1 may be an adequate target to inhibit breast cell proliferation.

Citations

Dec 14, 2002·Brain Research. Molecular Brain Research·Begoña SanchezRoman Perez-Fernandez
Nov 10, 2010·The Journal of Clinical Investigation·Isabel Ben-BatallaRoman Perez-Fernandez
Oct 8, 2011·Molecular Endocrinology·Yunguang TongShlomo Melmed
May 17, 2011·Indian Journal of Pharmacology·Chandra Kanti Chakraborti
Aug 3, 2005·European Journal of Endocrinology·C Gil-PuigR Perez-Fernandez
Mar 14, 2002·Biochemical and Biophysical Research Communications·Samuel SeoaneRomán Pérez-Fernández
May 9, 2007·Molecular and Cellular Endocrinology·Peter J AnsellAnne Klibanski
Jan 13, 2018·Oncogene·Anxo Martinez-OrdoñezRoman Perez-Fernandez
Dec 28, 2007·The Journal of Clinical Endocrinology and Metabolism·Mara GiordanoPatricia Momigliano-Richiardi
Jul 23, 2009·The Journal of Clinical Endocrinology and Metabolism·Maria Chiara ZatelliEttore degli Uberti
Nov 1, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Zhongcheng GaoMeng Wei
Jan 1, 2011·Expert Review of Endocrinology & Metabolism·Yash ChhabraAndrew J Brooks

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