Pit2 assemblies at the cell surface are modulated by extracellular inorganic phosphate concentration

Journal of Virology
C SalaünJ M Heard

Abstract

Pit2 is a type III sodium-dependent phosphate transporter and the cell surface receptor for amphotropic murine leukemia virus. Indirect arguments have previously suggested that retrovirus receptor assembly play a role in triggering membrane fusion. Using CHO cells expressing physiological amounts of functional versions of human Pit2 fused to various tagging epitopes, we provide evidence that Pit2 forms assemblies at the cell surface. Living cells were exposed to cross-linking reagents and protein extracts were treated with trifluoroacetic acid (TFA), a chemical that destroys all protein interactions but covalent links. Assemblies were also detected in the absence of cross-linking and TFA treatment, indicating that they are partially resistant to detergent denaturation. The formation of homo-oligomers was documented by the coimmunoprecipitation of differently tagged molecules. The amounts of Pit2 assemblies detected in the presence or in the absence of cross-linking reagents varied with extracellular inorganic phosphate concentration ([P(i)]). Variation of signal intensity was in the range of twofold, occurred in the absence of de novo protein synthesis and took place at the cell surface. These results indicate that Pit2 assembl...Continue Reading

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Citations

May 2, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Omar D PerezDouglas J Jolly
Sep 8, 2010·The Journal of Biological Chemistry·Christine SalaünGérard Friedlander
Nov 17, 2007·The Journal of Infectious Diseases·Anthony SiauDominique Mazier
Dec 23, 2006·European Journal of Oral Sciences·Dawei ZhaoAmel Gritli-Linde
Mar 20, 2013·British Journal of Clinical Pharmacology·Navid ShobeiriRachel M Holden
Oct 31, 2007·Biotechnology and Bioengineering·Natalia Landázuri, Joseph M Le Doux
Jun 22, 2007·American Journal of Physiology. Renal Physiology·Leila V VirkkiIan C Forster
Nov 27, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pascal FreiBruno Stieger
Jan 21, 2021·International Journal of Molecular Sciences·Joie L BehrensAlexandra S Muscher-Banse

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