Pitfalls encountered during quantitative determination of 3-alkyl-2-methoxypyrazines in grape must and wine using gas chromatography-mass spectrometry with stable isotope dilution analysis. Comprehensive two-dimensional gas chromatography-mass spectrometry and on-line liquid chromatography-multidimensional gas chromatography-mass spectrometry as potential loopholes

Journal of Chromatography. a
Hans-Georg SchmarrMaria Kutyrev

Abstract

The analysis of 3-alkyl-2-methoxypyrazines in Vitis vinifera grape must or wine at the low nanogram per liter level failed in several situations when applying a one-dimensional gas chromatographic analysis with mass spectrometric detection (GC-MS). Sample preparation methods such as headspace solid phase microextraction or solid phase extraction were convenient procedures, however lacking extraction selectivity for complex matrices. Analysis by comprehensive two-dimensional gas chromatography with mass spectrometric detection clearly demonstrated the potential for co-elution in such matrices and the risk for erroneous results when applying one-dimensional GC-MS. In one example, matrix problems would have been a challenge even for a comprehensive two-dimensional chromatographic approach with MS detection (GC×GC-MS). A solution to matrix problems was found by protonating the 3-alkyl-2-methoxypyrazines in acidic pH and sample clean-up using solid phase extraction with a mixed-mode polymeric cation-exchange sorbent. Quantification was performed by a stable isotope dilution assay, following analysis by on-line coupled high performance liquid chromatography with multidimensional gas chromatography and detection with mass spectrometry...Continue Reading

References

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Sep 17, 2009·Journal of Agricultural and Food Chemistry·Imelda RyonaGavin L Sacks
Oct 8, 2009·Journal of Agricultural and Food Chemistry·Philippus AlbertsAndre de Villiers

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Citations

May 17, 2011·Analytical and Bioanalytical Chemistry·Carolina Muñoz-GonzálezM Ángeles Pozo-Bayón
Sep 16, 2015·Journal of Chromatography. a·R M CallejónA M Troncoso
Nov 12, 2013·Rapid Communications in Mass Spectrometry : RCM·Johannes LangenHans-Georg Schmarr
Oct 31, 2014·Analytical and Bioanalytical Chemistry·Chiara CorderoCarlo Bicchi
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Davinder SidhuCedric Saucier
Jan 24, 2017·Journal of Agricultural and Food Chemistry·Chiara CorderoCarlo Bicchi
Dec 7, 2014·Analytica Chimica Acta·Sung-Tong Chin, Philip J Marriott
Dec 1, 2017·Critical Reviews in Analytical Chemistry·Tomasz Dymerski
Oct 3, 2018·Food Analytical Methods·Thomas SchneiderDavid R Goodlett
May 30, 2012·European Journal of Mass Spectrometry·Vincenzo SciontiChrys Wesdemiotis
May 17, 2014·Chemical Communications : Chem Comm·Sung-Tong Chin, Philip J Marriott
Nov 28, 2020·Journal of Separation Science·Juliane Elisa WelkeClaudia Alcaraz Zini

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