Pitfalls in the diagnosis of latex allergy

Allergology International : Official Journal of the Japanese Society of Allergology
Sujoy KhanCarrock Sewell

Abstract

Screening patients for latex allergy prior to surgery is an important but intensive procedure. The appropriate testing strategy for diagnosing latex (Hevea brasiliensis) allergy involves in-vitro specific IgE or skin prick testing. The sensitivity and specificity of both tests are influenced by patient-specific factors or manufacturing processes that alter the clinically relevant allergens in skin testing solutions. Total IgE and latex-specific IgE testing was introduced as a screening test. Skin prick testing was done on patients with a high probability of latex allergy and negative specific IgE with total IgE <100 kU/L. SDS-PAGE was done on the non-ammoniated latex (NAL) and newly introduced ammoniated latex (AL) reagents for the clinically relevant allergens. 51 patients had a total IgE <100 (range, 2.8-99.0 kU/L), and 10% had a positive skin test. 60% of positive skin tests would have been missed with lower total IgE cut-offs of 50 kU/L (6% of referrals). SDS-PAGE of the NAL solution showed 3 prominent bands with molecular weights of approximately 20, 24 and 42 kDa that correlated with Hev b 6, Hev b 3 and Hev b 7/13, respectively. In contrast, the AL solution showed 3 very faint higher molecular weights bands that did not ...Continue Reading

References

Apr 1, 1992·Electrophoresis·T RabilloudC Ettori
Oct 29, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·M ToraasonD Germolec
Sep 14, 2002·Journal of Immunoassay & Immunochemistry·Vesna J Tomazic-Jezic, Wava Truscott
Mar 17, 2004·Current Opinion in Allergy and Clinical Immunology·Hoong-Yeet Yeang
Jan 31, 2006·Journal of Clinical Pathology·W S SmellieC A Bloxham
Jul 13, 2007·International Journal of Immunopathology and Pharmacology·R BernardiniA Vierucci
Dec 17, 2008·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·S KhanW A C Sewell
Apr 30, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A VultaggioE Maggi

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Citations

Aug 2, 2019·Human Vaccines & Immunotherapeutics·Sarah M BakerLisa A Morici

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