PMID: 8982046Dec 1, 1996Paper

Pitfalls in the immunophenotyping of leukaemia and leukaemic lymphomas: survey of 9 years of quality control in The Netherlands. Dutch Cooperative Study Group on Immunophenotyping of Haematological Malignancies (SIHON)

British Journal of Haematology
J Kluin-NelemansJ Gratama

Abstract

During the last decade, biannual quality controls were performed in the Netherlands focusing on the immunophenotyping of leukaemic haematological malignancies. All results on 48 specimens obtained by 18-34 laboratories were analysed. The interlaboratory variability and percentages of discordant results from 30 markers were measured by assessing false positive or negative (cut-off 10%) results in comparison with median results of the group. The quality of the immunophenotypic diagnoses obtained from the interpretation of these markers in relation to clinical data was evaluated by scoring them as 'correct', 'minor fault', 'major fault', 'not based upon the markers used', and 'no diagnosis', CD3, CD8, CD19, CD61 and Sm lambda had the lowest percentage discordancy (sum of total negative and positive discordant values 5-7.5% of assays): CD13, CD15, cyCD22, CD33 and TdT scored worst with 14-20% cumulative discordancy. The analysis of each diagnosis yielded 78% acceptable immunophenotypic conclusions (correct 54% and minor fault 24%). It appeared that the major faults in immunophenotyping were caused by suboptimal antibody selection and erroneous interpretation of the results obtained, rather than by technical errors. Large difference...Continue Reading

Citations

Feb 13, 2001·British Journal of Haematology·J Kluin-NelemansUNKNOWN Dutch Co-operative Study Group on Immunophenotyping of Haematological Malignancies (SIHON)
Aug 17, 1999·Clinical and Laboratory Haematology·J W GratamaM B Van 't Veer
Aug 26, 2014·Diagnostic Pathology·Tawatchai PongpruttipanPongsak Wannakrairot
Nov 22, 2012·European Journal of Haematology·Giovanni RossiNicola Cascavilla
Dec 21, 2006·Cytometry. Part B, Clinical Cytometry·Bruno BrandoJan Willem Gratama
Nov 10, 2005·Cytometry. Part B, Clinical Cytometry·Richard RateiUNKNOWN European Working Group of Clinical Cell Analysis (EWGCCA)
Jun 24, 2015·Cytometry. Part B, Clinical Cytometry·Frank W M B PreijersUNKNOWN Section Immunological and Molecular Cell Diagnostics of the Foundation for Quality Assessment in Medical Laboratories(SKML)

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