Pitfalls in the measurement of metabolite concentrations using the one-pulse experiment in in Vivo NMR: commentary on "On neglecting chemical exchange effects when correcting in vivo (31)P MRS data for partial saturation"

Journal of Magnetic Resonance
Richard G S SpencerCraig J Galbán

Abstract

In an article in a previous issue of the Journal of Magnetic Resonance, Ouwerkerk and Bottomley (J. Magn. Reson. 148, pp. 425--435, 2001) show that even in the presence of chemical exchange, the dependence of saturation factors on repetition time in the one-pulse experiment is approximately monoexponential. They conclude from this fact that the effect of chemical exchange on the use of saturation factors when correcting for partial saturation is negligible. We take issue with this conclusion and demonstrate that because saturation factors in the presence of chemical exchange are strongly dependent upon all of the chemical parameters of the system, that is, upon all T(1)'s and M(0)'s of resonances in the exchange network and upon the reaction rates themselves, it is problematic to apply saturation factor corrections in situations in which any of these parameters may change. The error criterion we establish reflects actual errors in quantitation, rather than departures from monoexponentiality.

References

Dec 1, 1991·Journal of Molecular and Cellular Cardiology·R Kalil-FilhoR G Weiss
Mar 7, 1996·Nature·K H CampbellI Wilmut
Aug 1, 1996·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·A Horská, R G Spencer
Feb 29, 2000·The Journal of Biological Chemistry·J H Cieslar, G P Dobson
Mar 10, 2001·Journal of Magnetic Resonance·R Ouwerkerk, P A Bottomley

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Citations

Nov 22, 2016·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jimin RenCraig R Malloy
Jul 31, 2007·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Craig J Galbán, Richard G Spencer
Feb 23, 2019·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jimin RenCraig R Malloy
Nov 13, 2009·NMR in Biomedicine·Brent P ForesterBruce M Cohen
Aug 27, 2010·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Damian J TylerRichard G Spencer
May 27, 2015·NMR in Biomedicine·Petronella A van EwijkM Eline Kooi
Apr 23, 2002·NMR in Biomedicine
Sep 5, 2002·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Sebastian KozerkePeter Boesiger

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