Pituispheres Contain Genetic Variants Characteristic to Pituitary Adenoma Tumor Tissue.

Frontiers in Endocrinology
Raitis PeculisVita Rovite

Abstract

The most common type of pituitary neoplasms is benign pituitary adenoma (PA). Clinically significant PAs affect around 0.1% of the population. Currently, there is no established human PA cell culture available and when PA tumor cells are cultured they form two distinct types depending on culturing conditions either free-floating aggregates also known as pituispheres or cells adhering to the surface of cell plates and displaying mesenchymal stem-like properties. The aim of this study was to trace the origin of sphere-forming and adherent pituitary cell cultures and characterize the potential use of these surgery derived cell lines as PA model. We carried out a paired-end exome sequencing of patients' tumor and germline DNA using Illumina NextSeq followed by characterization of corresponding PA cell cultures. Variation analysis revealed a low amount of somatic mutations (mean = 5.2, range 3-7) in exomes of PAs. Somatic mutations of the primary surgery material can be detected in the exomes of respective pituispheres, but not in exomes of respective mesenchymal stem-like cells. For the first time, we show that the genome of pituispheres represents genome of PA while mesenchymal stem cells derived from the PA tissue do not contain ...Continue Reading

Associated Datasets

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Datasets Mentioned

BETA
PRJNA623684

Methods Mentioned

BETA
surgical resection
biopsies
xenografts
Assay
Exome Sequencing
RNA-seq

Software Mentioned

Ensembl
Leica Application Suite X ( LAs X )
Illumina Basespace Enrichment App
Illumina Annotation Engine
PolyPhen
Starling
Genera
Isaac
SIFT
ExAc

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