Pituitary adenylate cyclase-activating polypeptide and cyclic adenosine 3',5'-monophosphate stimulate the promoter activity of the rat gonadotropin-releasing hormone receptor gene via a bipartite response element in gonadotrope-derived cells

The Journal of Biological Chemistry
H PincasR Counis

Abstract

Specific type I receptors for pituitary adenylate cyclase-activating polypeptide (PACAP) are present in gonadotrope cells of the anterior pituitary gland. By transient transfection of mouse gonadotrope-derived alphaT3-1 cells, which are direct targets for PACAP and express gonadotropin-releasing hormone receptor (GnRH-R), a marker of the gonadotrope lineage, we provide the first evidence that PACAP stimulates rat GnRH-R gene promoter activity. The EC(50) of this stimulation is compatible with a mediation via activation of the cyclic AMP-dependent signaling pathway and, consistently, co-transfection of an expression vector expressing the protein kinase A inhibitor causes reduction in PACAP as well as cholera toxin-stimulated promoter activity. Deletion and mutational analyses indicate that PACAP activation necessitates a bipartite response element that consists of a first region (-272/-237) termed PACAP response element (PARE) I that includes a steroidogenic factor-1 (SF-1)-binding site and a second region (-136/-101) referred to as PARE II that contains an imperfect cyclic AMP response element. Gel shift experiments indicate the specific binding of the SF-1 and a potential SF-1-interacting factor to PARE I while a protein immun...Continue Reading

References

Oct 16, 1989·Biochemical and Biophysical Research Communications·A MiyataD H Coy
Mar 21, 1998·The Journal of Biological Chemistry·D MontéD W Hum

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Citations

Mar 9, 2012·Journal of Molecular Neuroscience : MN·Gal Levy, Gad Degani
Dec 31, 2010·Biology of Reproduction·Stephen J Winters, Joseph P Moore
Sep 11, 2013·Journal of Molecular Endocrinology·Robin L ThomasLisa M Halvorson
Jul 3, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Stephen J Winters, Joseph P Moore
Oct 8, 2013·Molecular and Cellular Endocrinology·Lisa M Halvorson
Jan 21, 2014·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Brian EdwardsDonal C Skinner
Jul 3, 2007·Peptides·Raymond CounisSolange Magre
Sep 15, 2005·Journal of Neuroendocrinology·J P HapgoodK Ronacher
Jul 17, 2013·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Gal Levy, Gad Degani
Aug 30, 2016·Molecular and Cellular Endocrinology·Ivana BjelobabaStanko S Stojilkovic
Jun 27, 2013·Molecular Endocrinology·Constance M Grafer, Lisa M Halvorson
May 16, 2014·Endocrinology·Stephen J WintersJoseph P Moore
Jun 9, 2016·Molecular Endocrinology·Alice K TreenDenise D Belsham
Jul 18, 2018·Reproductive Medicine and Biology·Aki OrideSatoru Kyo
Apr 4, 2021·International Journal of Molecular Sciences·Angela K OdleAngus M MacNicol
Jun 21, 2020·Molecular and Cellular Endocrinology·Stephen J Winters, Joseph P Moore

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