PMID: 8959742Jan 1, 1996Paper

Pituitary adenylate cyclase-activating polypeptide (PACAP-38) stimulates rat peritoneal macrophage functions

Peptides
M DelgadoR P Gomariz

Abstract

The present study shows that PACAP-38, in a dose-dependent manner, increased in vitro two steps of the phagocytic process in rat peritoneal macrophages: ingestion of inert particles (latex beads) and production of superoxide anion as measured by nitroblue tetrazolium reduction. The most effective concentration of PACAP-38 was 10(-10) M. Similarly, PMA, an activator of protein kinase C (PKC), increased the phagocytic activity in a dose-dependent manner, whereas retinal, a PKC inhibitor, decreased the activity. Macrophages incubated with forskolin, an enhancer of intracellular cAMP levels, produced an inhibitory effect on both phagocytic functions. The maximum stimulation of the phagocytic activity by PACAP-38 was not further enhanced by addition of PMA, suggesting that the enhancement of the phagocytic activity by PACAP-38 and PMA is mediated by a common signaling pathway. In addition, retinal as well as forskolin inhibited partially the stimulatory effect that PACAP-38 produced in the macrophage functions studied. Furthermore, this study showed that a VIP receptor antagonist was unable to suppress the stimulatory effect of PACAP-38. These results could prove that PACAP-38 stimulates the phagocytosis and production of superoxide...Continue Reading

References

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Citations

Nov 10, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H D LauensteinD A Groneberg
Jun 10, 2003·Annals of the New York Academy of Sciences·Mario DelgadoRosa P Gomariz
Aug 27, 2010·Expert Opinion on Biological Therapy·Lucília da SilvaMaria Teresa Cruz
Sep 29, 1999·Pulmonary Pharmacology & Therapeutics·L Lindén
Oct 27, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Christoph W MichalskiHelmut Friess
Sep 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C AbadM Delgado
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Issam HarfiEric Sariban
Jun 14, 2019·Frontiers in Cellular and Infection Microbiology·Caio Andreeta FigueiredoIldiko Rita Dunay

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