PMID: 9523583Apr 2, 1998Paper

Pituitary adenylate cyclase-activating polypeptide causes Ca2+ release from ryanodine/caffeine stores through a novel pathway independent of both inositol trisphosphates and cyclic AMP in bovine adrenal medullary cells

Journal of Neurochemistry
K TanakaH Yamashita

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) causes both Ca2+ release and Ca2+ influx in bovine adrenal chromaffin cells. To elucidate the mechanisms of PACAP-induced Ca2+ release, we investigated expression of PACAP receptors and measured inositol trisphosphates (IP3), cyclic AMP, and the intracellular Ca2+ concentration in bovine adrenal medullary cells maintained in primary culture. RT-PCR analysis revealed that bovine adrenal medullary cells express the PACAP receptor hop, which is known to couple with both IP3 and cyclic AMP pathways. The two naturally occurring forms of PACAP, PACAP38 and PACAP27, both increased cyclic AMP and IP3, and PACAP38 was more potent than PACAP27 in both effects. Despite the effects of PACAP on IP3 production, the Ca2+ release induced by PA-CAP38 or by PACAP27 was unaffected by cinnarizine, a blocker of IP3 channels. The potencies of the peptides to cause Ca2+ release in the presence of cinnarizine were similar. The Ca2+ release induced by PACAP38 or by PACAP27 was strongly inhibited by ryanodine and caffeine. In the presence of ryanodine and caffeine, PACAP38 was more potent than PACAP27. PACAP-induced Ca2+ release was unaffected by Rp-adenosine 3',5'-cyclic monophosphothioate, an ...Continue Reading

Citations

Jan 14, 1999·The American Journal of Physiology·S LamoucheN Yamaguchi
Jun 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·D J TurnerM W Mulholland
May 25, 2002·The European Journal of Neuroscience·Sacha McKenzie, Philip D Marley
Dec 22, 1999·British Journal of Pharmacology·P J BalesP D Marley
Jul 13, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Y FukushimaS Satoh
Apr 2, 2003·Pharmacology & Therapeutics·Philip D Marley
Apr 28, 2004·Autonomic Neuroscience : Basic & Clinical·Hideaki KamaishiTakashi Suzuki
Nov 16, 2004·Cell Calcium·Naoko SasakiIzumi Shibuya

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