PMID: 9651104Jul 3, 1998Paper

Pituitary adenylate cyclase-activating polypeptide (PACAP) 38 and PACAP27 differentially stimulate growth hormone release and mRNA accumulation in porcine somatotropes

Life Sciences
A J Martínez-FuentesF Gracia-Navarro

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) has been suggested to regulate growth hormone (GH) secretion in several species. Here, we analyzed the in vitro effects of PACAP38 and PACAP27 on the secretory activity of porcine somatotropes. Cultures of porcine pituitary cells were treated with PACAP38 and PACAP27, and GH release, intracellular GH content, and GH mRNA levels were evaluated. Also, the time course of changes in the somatotrope content of GH and its mRNA in response to PACAPs were measured. Both PACAPs stimulated GH release from porcine somatotropes in a broad range of doses (10(-10)-10(-6) M), yet only PACAP27 elicited a dose-dependent response. GH cell content remained essentially unchanged after PACAP treatment. In contrast, both PACAPs induced significant and sustained increases in GH mRNA cell content, although the response to PACAP27 appeared faster (8 h) than to PACAP38 (16 h). These results demonstrate that PACAP stimulates GH production in porcine somatotropes. Furthermore, the differential responses induced by PACAP38 and PACAP27 suggest that distinct mechanisms mediate their effects on this cell type.

References

Oct 16, 1989·Biochemical and Biophysical Research Communications·A MiyataD H Coy
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Oct 1, 1994·The Journal of Endocrinology·B VelkeniersE L Hooghe-Peters
Jun 1, 1993·Journal of Neuroendocrinology·R TorronterasF Gracia-Navarro
Jun 1, 1993·Brain : a Journal of Neurology·A FeinsteinA Thompson

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Citations

May 9, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·F Gracia-NavarroA J Martínez-Fuentes
May 22, 2009·Annals of the New York Academy of Sciences·Manuel D GaheteJusto P Castaño
Jul 11, 2009·Endocrinology·Francisca Rodríguez-PachecoMaria M Malagón
Mar 27, 2004·Experimental Biology and Medicine·Lloyd L AndersonColin G Scanes
Jun 2, 2005·Domestic Animal Endocrinology·L L AndersonA Glavaski-Joksimovic

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