Pituitary and brain 5alpha-reductase messenger RNA levels in control, castrated, and dihydrotestosterone-treated rats

Molecular and Cellular Neurosciences
E D Lephart

Abstract

In (28- to 40-day-old) male rats, the influence of castration or hormone-replacement treatment with dihydrotestosterone (5alpha-DHT) upon pituitary or hypothalamic 5alpha-reductase was assessed. The efficacy of the treatments was demonstrated by measuring serum LH and ventral prostate weight. Pituitary 5alpha-reductase enzyme activity was estimated by isolating [5alpha-(3)H]DHT by thin-layer chromatography after incubations with [(3)H]testosterone, and the identity of the [5alpha-(3)H]DHT formed was confirmed by recrystallization experiments. Pituitary and hypothalamic 5alpha-reductase mRNA content was determined by RNA blot analysis utilizing a rat 5alpha-reductase (type 1 or type 2) cDNA as probe. Pituitary 5alpha-reductase activity was significantly increased as a function of days after castration, whereas castration plus DHT treatment (at Day 12 post-castration) significantly decreased the activity to less than one-third of control levels. In controls, pituitary 5alpha-reductase mRNA content was barely detectable using the rat 5alpha-reductase (type 1) cDNA as probe. However, castration resulted in a clear increase in mRNA abundance, while in DHT-injected castrated animals pituitary mRNA content was undetectable. Hypothalam...Continue Reading

Citations

May 30, 2014·The Journal of Endocrinology·Dawn E W LivingstoneRuth Andrew
Oct 26, 2014·Naunyn-Schmiedeberg's Archives of Pharmacology·Lorena SuárezManuel Sánchez
Sep 10, 2011·The Journal of Endocrinology·Mark NixonRuth Andrew
Nov 2, 2005·The Journal of Comparative Neurology·Emi KiyokageKazunori Ishimura
Oct 13, 1998·The Journal of Clinical Endocrinology and Metabolism·B Stoffel-WagnerH U Schweikert
Dec 18, 2001·Brain Research. Brain Research Reviews·E D LephartT L Horvath
Jan 1, 1997·Brain Research Bulletin·F CelottiA Poletti

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