Pituitary and plasma lipotropins: demonstration of the artificial nature of betaMSH

The Journal of Clinical Endocrinology and Metabolism
I BachelotW D Odell

Abstract

Evidence has been presented by others that betaMSH immunoreactivity in human plasma is due to beta and gamma lipotropin rather than betaMSH. We have studied this question in normal subjects utilizing a sensitive human betaMSH radioimmunoassay capable of quantifying betaMSH in unextracted plasma with a sensitivity of 7.5 pg/ml. Purified human beta lipotropin cross-reacted 30% on a molar basis with synthetic human betaMSH in this assay. ACTH related peptides showed less than 0.1% cross-reactivity. Normal values at 0800 h were 19.6+/-2.4 pg/ml and suppressed to 9.3+/-1.3 pg/mol following dexamethasone. Metyrapone increased 0800 h values to 379.6+/-89.9 pg/ml. Chromatographic studies on Sephadex G-50 demonstrated no betaMSH per se in human pituitaries, plasma from metyrapone treated normals, patients with Cushing's disease. Nelson's syndrome, or Addison's disease. betaMSH immunoreactivity showed the elution pattern of beta lipotropin.

Citations

Aug 11, 1980·Life Sciences·G E FeurleV Helmstaedter
May 1, 1982·Peptides·T L O'Donohue, D M Dorsa
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E Saito, W D Odell
Jul 1, 1978·The Journal of Clinical Investigation·K TanakaD N Orth
Apr 1, 1979·The American Journal of Medicine·W D OdellF M Hirose
May 1, 1980·Clinical Endocrinology·R A Donald
Jul 1, 1982·International Journal of Dermatology·P Agache, D Blanc
Apr 1, 1985·The Journal of Dermatology·J J Nordlund
Jan 28, 1981·Molecular and Cellular Biochemistry·M Chrétien, N G Seidah
Jan 1, 1981·International Journal of Peptide and Protein Research·E W Van CauterG Copinschi
May 1, 1979·The American Journal of Medicine·A R Wolfsen, W D Odell

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