PMID: 8604340Mar 15, 1996Paper

Pituitary-specific chromatin structure of the rat prolactin distal enhancer element

Nucleic Acids Research
S D Willis, M A Seyfred

Abstract

The location of target DNA sequences within chromatin may affect the ability of trans-acting factors to bind cis-elements and regulate gene transcription. To examine the effect of chromatin structure on the ability of the estrogen-estrogen receptor complex (E2R) to bind its respective DNA binding element within the rat prolactin (rPRL) gene and modulate rPRL gene expression, we have developed cell lines derived from the rPRL-expressing (rPRL+) rat pituitary cell line GH3 and the rPRL-non- expressing (rPRL-) rat embryo fibroblast cell line Rat1. These cell lines contain mini-chromosomes composed of the 5' upstream regulatory region of the rPRL gene driving expression of a reporter gene, Tn5, within a bovine papillomavirus (BPV) vector. The rPRL-Tn5 gene retains the characteristics of cell-specific expression and estrogen inducibility of transcription displayed by the endogenous rPRL gene. The distal enhancer region, which contains an estrogen response element, was found to exist in a nucleosome-free region in pituitary-derived cells even in the absence of estrogen. In contrast, the rPRL distal enhancer in fibroblast cells was found to be randomly packaged into nucleosomes. These results indicate that DNA sequence is not sufficie...Continue Reading

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K D Carr, H Richard-Foy
Feb 1, 1989·Molecular Endocrinology·M A SeyfredJ Gorski
Dec 1, 1994·Molecular Endocrinology·B E Nowakowski, R A Maurer
Apr 1, 1994·Molecular and Cellular Endocrinology·D Gourdji, J N Laverrière

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Citations

May 14, 2003·Chromosoma·Pratibha B Hebbar, Trevor K Archer
Jun 17, 2000·The International Journal of Biochemistry & Cell Biology·A Kim, V Murray
Oct 19, 2001·The International Journal of Biochemistry & Cell Biology·A R Kim, V Murray
Mar 18, 2003·Inquiry : a Journal of Medical Care Organization, Provision and Financing·Jennifer D Uhrig, Pamela Farley Short
Sep 14, 2006·Journal of Cell Science·Z Dave SharpMichael A Mancini

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