Abstract
Although engineered cardiac tissues (ECTs) derived from induced pluripotent stem cells (iPSCs) are promising for myocardial regenerative therapy, the appropriate ratio of cardiomyocytes to non-cardiomyocytes is not fully understood. Here, we determined whether ECT-cell content is a key determinant of its structure/function, thereby affecting ECT therapeutic potential for advanced heart failure. Scaffold-free ECTs containing different ratios (25%, 50%, 70%, or 90%) of iPSC-derived cardiomyocytes were generated by magnetic-activated cell sorting by using cardiac-specific markers. Notably, ECTs showed synchronized spontaneous beating when cardiomyocytes constituted ≥50% of total cells, with the electrical-conduction velocity increasing depending on cardiomyocyte ratio; however, ECTs containing 90% cardiomyocytes failed to form stable structures. ECTs containing 25% or 50% cardiomyocytes predominantly expressed collagen and fibronectin, whereas ECTs containing 70% cardiomyocytes predominantly expressed laminin and exhibited the highest contractile/relaxation properties. Furthermore, transplantation of ECTs containing 50% or 70% cardiomyocytes into a rat chronic myocardial infarction model led to a more profound functional recovery ...Continue Reading
References
Jun 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bertrand LeobonSerge Charpak
Feb 8, 2006·Annual Review of Physiology·Patrick C H HsiehRichard T Lee
May 17, 2006·Acta Biomaterialia·Hong-Fang LuHai-Quan Mao
Mar 19, 2011·Circulation Research·Adam R WilliamsJoshua M Hare
May 27, 2011·Stem Cell Reviews and Reports·Yee-Ki LeeChung-Wah Siu
Aug 3, 2011·Biomaterials·Katsuhisa MatsuuraTeruo Okano
Aug 31, 2011·Tissue Engineering. Part a·Hidekazu SekineTeruo Okano
Oct 20, 2011·Journal of Molecular and Cellular Cardiology·P CollierJ A Baugh
Oct 25, 2011·Nature Biotechnology·Nicole C DuboisGordon Keller
Dec 15, 2011·Physiological Genomics·Jane SynnergrenPeter Sartipy
Jul 31, 2012·Biochemical and Biophysical Research Communications·Katsuhisa MatsuuraTeruo Okano
Sep 22, 2012·Circulation·Masashi KawamuraYoshiki Sawa
Dec 1, 2012·Stem Cells Translational Medicine·Kenji MikiYoshiki Sawa
Sep 10, 2013·Acta Biomaterialia·C WilliamsL D Black
Jan 5, 2014·Circulation Research·Elena MatsaJoseph C Wu
Feb 1, 2014·Circulation Research·Xiulan YangCharles E Murry
Apr 12, 2014·Circulation Research·Pratik A LalitTimothy J Kamp
May 20, 2014·Current Treatment Options in Cardiovascular Medicine·Gwanghyun Jung, Daniel Bernstein
Sep 27, 2014·Journal of Molecular and Cellular Cardiology·Tomohiro HayakawaTetsushi Furukawa
Nov 26, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Satoshi KainumaYoshiki Sawa
Apr 22, 2015·Cell Stem Cell·Takanori TakebeHideki Taniguchi
Aug 5, 2015·The Journal of Biological Chemistry·Julianne BeamSanjoy Ghosh
Citations
Aug 1, 2018·Expert Opinion on Biological Therapy·Tilman ZieglerChristian Kupatt
Oct 3, 2017·Tissue Engineering. Part C, Methods·Maki TakedaYoshiki Sawa
Jul 7, 2018·Circulation Research·Jianyi ZhangGordana Vunjak-Novakovic
Aug 18, 2018·Transplantation·Masaru IshidaYoshiki Sawa
Jan 20, 2019·Biotechnology and Bioengineering·Bernardo AbecasisPaula M Alves
Mar 16, 2019·Scientific Reports·Fumiya OhashiYoji Sato
Mar 26, 2019·Cellular Reprogramming·Mingyu WangXiong Xiao
Dec 13, 2018·Current Drug Targets·Xiaotong WangYe Yuan
Oct 9, 2019·Biofabrication·Jonathan R SoucyRyan A Koppes
Nov 21, 2019·Stem Cells and Development·Sherri M Biendarra-TiegsTimothy J Nelson
Mar 1, 2020·Tissue Engineering. Part C, Methods·Diwakar TuragaTodd C McDevitt
May 11, 2018·Nature Reviews. Cardiology·Philippe Menasché
Sep 21, 2018·Tissue Engineering. Part C, Methods·Katsuhisa MatsuuraTatsuya Shimizu
Nov 9, 2018·Frontiers in Cardiovascular Medicine·Maedeh ZamaniNgan F Huang
May 10, 2019·APL Bioengineering·Neal I CallaghanCraig A Simmons
Aug 20, 2017·Scientific Reports·Masashi KawamuraYoshiki Sawa
Aug 14, 2020·Journal of the American Heart Association·Takaaki SamuraYoshiki Sawa
Oct 11, 2020·Acta Pharmacologica Sinica·Xiang WangLu Cai
Dec 2, 2020·International Journal of Molecular Sciences·Junjun LiYoshiki Sawa
Nov 17, 2020·Frontiers in Cardiovascular Medicine·Dillon K JarrellJeffrey G Jacot
Nov 17, 2020·Frontiers in Bioengineering and Biotechnology·Philippe Menasché
Mar 5, 2021·Frontiers in Cell and Developmental Biology·Jun LiYasushi Sakata
Apr 4, 2021·International Journal of Molecular Sciences·Aleksandra NijakMaaike Alaerts
Oct 16, 2020·Bioscience Reports·Peng WuPing Zhu
Jun 22, 2021·Frontiers in Bioengineering and Biotechnology·Mira ChingaleKe Huang
Jun 15, 2021·Stem Cell Research·Hiroko IseokaYoshiki Sawa
Jul 21, 2021·Scientific Reports·Kiyoshi MasuyamaYasushi Sakata
Aug 17, 2021·Journal of Biomedical Materials Research. Part a·Xinyue ChuXiong Xiao
Nov 27, 2021·Physiological Reports·Mohamadamin ForouzandehmehrMichelangelo Paci