Piwi Is a Key Regulator of Both Somatic and Germline Stem Cells in the Drosophila Testis

Cell Reports
Jacob GonzalezHaifan Lin

Abstract

The Piwi-piRNA pathway is well known for its germline function, yet its somatic role remains elusive. We show here that Piwi is required autonomously not only for germline stem cell (GSC) but also for somatic cyst stem cell (CySC) maintenance in the Drosophila testis. Reducing Piwi activity in the testis caused defects in CySC differentiation. Accompanying this, GSC daughters expanded beyond the vicinity of the hub but failed to differentiate further. Moreover, Piwi deficient in nuclear localization caused similar defects in somatic and germ cell differentiation, which was rescued by somatic Piwi expression. To explore the underlying molecular mechanism, we identified Piwi-bound piRNAs that uniquely map to a gene key for gonadal development, Fasciclin 3, and demonstrate that Piwi regulates its expression in somatic cyst cells. Our work reveals the cell-autonomous function of Piwi in both somatic and germline stem cell types, with somatic function possibly via its epigenetic mechanism.

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Citations

Feb 11, 2016·International Journal of Molecular Sciences·Lihong ZhaiWenbin Deng
Feb 18, 2016·Stem Cells International·Shanshan Hu, Ge Shan
Jan 25, 2017·Nucleic Acids Research·Erika GirardiRonald P van Rij
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Jul 4, 2018·Molecular Reproduction and Development·Beibei BieJin Sun
Sep 13, 2017·Molecular Genetics and Genomics : MGG·Heeyoun Bunch
Mar 14, 2020·PLoS Genetics·Zsolt G VenkeiYukiko M Yamashita
May 7, 2020·RNA Biology·Charannya Sozheesvari SubhramanyamQidong Hu
Mar 10, 2016·The Journal of Biological Chemistry·Zifeng WangHaifan Lin
Dec 28, 2016·Molecular and Cellular Biochemistry·Suganya SivagurunathanSubbulakshmi Chidambaram
Feb 16, 2019·BMC Genetics·Sylvaine RenaultCorinne Augé-Gouillou
Sep 19, 2017·The Journal of Clinical Investigation·Gregory A WassermanMatthew R Jones
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Feb 21, 2021·G3 : Genes - Genomes - Genetics·Na LiuHaifan Lin
Feb 22, 2021·Developmental Biology·Velinda Vidaurre, Xin Chen

Related Concepts

Fas3 (fasciclin 3) protein, Drosophila
Piwi protein, Drosophila
Metazoa
Cell Differentiation Process
Cell Nucleus
Drosophila melanogaster
Y-Chromosome-Bearing Sperm
Colony-forming Unit
Testis
Cell Adhesion Molecules, Neuronal

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