The Piwi-piRNA pathway is well known for its germline function, yet its somatic role remains elusive. We show here that Piwi is required autonomously not only for germline stem cell (GSC) but also for somatic cyst stem cell (CySC) maintenance in the Drosophila testis. Reducing Piwi activity in the testis caused defects in CySC differentiation. Accompanying this, GSC daughters expanded beyond the vicinity of the hub but failed to differentiate further. Moreover, Piwi deficient in nuclear localization caused similar defects in somatic and germ cell differentiation, which was rescued by somatic Piwi expression. To explore the underlying molecular mechanism, we identified Piwi-bound piRNAs that uniquely map to a gene key for gonadal development, Fasciclin 3, and demonstrate that Piwi regulates its expression in somatic cyst cells. Our work reveals the cell-autonomous function of Piwi in both somatic and germline stem cell types, with somatic function possibly via its epigenetic mechanism.
Drosophila fasciclin I is a novel homophilic adhesion molecule that along with fasciclin III can mediate cell sorting
decapentaplegic is essential for the maintenance and division of germline stem cells in the Drosophila ovary
A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal
Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis
act up controls actin polymerization to alter cell shape and restrict Hedgehog signaling in the Drosophila eye disc
Yb modulates the divisions of both germline and somatic stem cells through piwi- and hh-mediated mechanisms in the Drosophila ovary
Rapid deadenylation triggered by a nonsense codon precedes decay of the RNA body in a mammalian cytoplasmic nonsense-mediated decay pathway
Regulatory relationship among piwi, pumilio, and bag-of-marbles in Drosophila germline stem cell self-renewal and differentiation
Zfh-1 controls somatic stem cell self-renewal in the Drosophila testis and nonautonomously influences germline stem cell self-renewal
The Yb body, a major site for Piwi-associated RNA biogenesis and a gateway for Piwi expression and transport to the nucleus in somatic cells.
Uniting germline and stem cells: the function of Piwi proteins and the piRNA pathway in diverse organisms
Somatic cell lineage is required for differentiation and not maintenance of germline stem cells in Drosophila testes
Transcriptional silencing of transposons by Piwi and maelstrom and its impact on chromatin state and gene expression
Drosophila piwi mutants exhibit germline stem cell tumors that are sustained by elevated Dpp signaling
Piwi is required in multiple cell types to control germline stem cell lineage development in the Drosophila ovary
Whole-animal genome-wide RNAi screen identifies networks regulating male germline stem cells in Drosophila
Rbf Regulates Drosophila Spermatogenesis via Control of Somatic Stem and Progenitor Cell Fate in the Larval Testis
Association of zygotic piRNAs derived from paternal P elements with hybrid dysgenesis in Drosophila melanogaster
Differential expression of piRNAs in reprogrammed pluripotent stem cells from mouse embryonic fibroblasts
Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis
Possible role of HIWI2 in modulating tight junction proteins in retinal pigment epithelial cells through Akt signaling pathway.
Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination
Glial granules contain germline proteins in the Drosophila brain, which regulate brain transcriptome
Homologues of Piwi control transposable elements and development of male germline in Penaeus monodon.
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