PIWI-like protein, HIWI2: A novel player in proliferative diabetic retinopathy.

Experimental Eye Research
Suganya SivagurunathanSubbulakshmi Chidambaram

Abstract

Diabetic retinopathy (DR) is one of the major causes of blindness resulting from prolonged hyperglycemia which leads to breakdown of blood retinal barrier and excessive neovascularization. In our previous study, we demonstrated the presence of germline-specific PIWI-like proteins in human retina and retinal pigment epithelium (RPE) and a discrete function of HIWI2 (PIWIL4) in the assembly of tight junction through Akt/GSK3α/β. Recently, PIWI/piRNA has been suggested to be involved in the development of diabetes. Here, we have investigated the role of HIWI2 in proliferative diabetic retinopathy (PDR). Interestingly, Western blot analysis indicated the elevated expression of HIWI2 in vitreous aspirates of patients with PDR in comparison to macular hole (MH) and rhegmatogenous retinal detachment (RRD). In addition, treatment of ARPE19 with 25% of PDR vitreous aspirate significantly increased the expression of HIWI2. Moreover, exposure of ARPE19 to oxidative stress and VEGF, induced the expression of HIWI2. Further, we knocked down HIWI2 in ARPE19 cells to understand its role in the disease progression. Silencing HIWI2 reduced the expression of growth factors, VEGF and TGFβ1, and altered the expression of epithelial to mesenchymal ...Continue Reading

Citations

Jun 20, 2019·International Journal of Molecular Sciences·Hassan MansoorGary Hin-Fai Yam

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Biomarkers for Diabetes

This feed focuses on the latest research on biomarkers used for monitoring disease progression in diabetes.