Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins

Nature Genetics
Jamy C PengHaifan Lin

Abstract

The Drosophila melanogaster Piwi protein regulates both niche and intrinsic mechanisms to maintain germline stem cells, but its underlying mechanism remains unclear. Here we report that Piwi interacts with Polycomb group complexes PRC1 and PRC2 in niche and germline cells to regulate ovarian germline stem cells and oogenesis. Piwi physically interacts with the PRC2 subunits Su(z)12 and Esc in the ovary and in vitro. Chromatin coimmunoprecipitation of Piwi, the PRC2 enzymatic subunit E(z), histone H3 trimethylated at lysine 27 (H3K27me3) and RNA polymerase II in wild-type and piwi mutant ovaries demonstrates that Piwi binds a conserved DNA motif at ∼ 72 genomic sites and inhibits PRC2 binding to many non-Piwi-binding genomic targets and H3K27 trimethylation. Moreover, Piwi influences RNA polymerase II activities in Drosophila ovaries, likely via inhibiting PRC2. We hypothesize that Piwi negatively regulates PRC2 binding by sequestering PRC2 in the nucleoplasm, thus reducing PRC2 binding to many targets and influencing transcription during oogenesis.

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Citations

Sep 11, 2016·Current Opinion in Plant Biology·Jun XiaoDoris Wagner
Oct 4, 2018·Critical Reviews in Biochemistry and Molecular Biology·Lyubov A LebedevaYulii V Shidlovskii
Feb 28, 2019·Genes & Development·Xiaolu ZhaoYifan Liu
Oct 17, 2017·The EMBO Journal·Patricia Rojas-RíosMartine Simonelig
Sep 9, 2018·Development·Patricia Rojas-Ríos, Martine Simonelig
Jan 31, 2018·Genetics·Ryan J GleasonXin Chen
Oct 27, 2017·Cancer Research·Chris Zhiyi ZhangJing-Ping Yun
Jan 7, 2021·Cell Reports·Haidong HuangJennifer S Yu
Feb 21, 2021·G3 : Genes - Genomes - Genetics·Na LiuHaifan Lin
Feb 22, 2021·Developmental Biology·Velinda Vidaurre, Xin Chen
Sep 30, 2020·Mechanisms of Development·Niloufer P Dumasia, Prasad S Pethe
Nov 16, 2021·International Journal of Oncology·Michaela Dostalova Merkerova, Zdenek Krejcik

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Methods Mentioned

BETA
co-immunoprecipitation
immunoprecipitation
ChIP-Seq
RNA-Seq
ChIP
pull down
methyltransferase assay

Software Mentioned

PANTHER Gene Ontology
SAM
ImageJ
MEME
R Development Core Team
QuEST peak caller
DNANexus
Novoalign read mapper
PANTHER
R

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