Pixel Counting of Fluorescence Spots Triggered by DNA Walkers for Ultrasensitive Quantification of Nucleic Acid

Analytical Chemistry
Longyi ZhuJianping Lei

Abstract

A pixel counting strategy is designed on the basis of DNA walker-triggered fluorescence spots for ultrasensitive detection of nucleic acid. The two-dimensional DNA walker was constructed by hybridization of two types of capture DNAs, which were covalently modified by click chemistry on a glass slide, and dye-labeled hairpin structure (hDNA) as track and swing strand (sDNA) as DNAzyme, respectively. Introduction of target DNA unlocked the sDNA via strand displacement to form the activated DNAzyme, and the latter cut nearby hDNA with Mn2+ as cofactor, resulting in fluorescence recovery of dye-labeled hDNA on the substrate due to the separation from the quencher. Meanwhile, the DNAzyme sequence of sDNA was released to cut the next hDNA and, thus, initiated autonomous walking of sDNA for signal amplification. The enhanced fluorescence spots were digitalized as pixels on the basis of DNA walker-built compartments and extracted by a homemade program in MATLAB. The association between fluorescent pixel numbers and DNA concentrations was further proved by a mathematical model and led to an ultrasensitive quantification of nucleic acid with a linear range from 100 fM to 10 pM. The designed pixel counting strategy shows a more sensitive ...Continue Reading

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Citations

Feb 19, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rashaad A HusainZong-Hong Lin
Jul 28, 2020·Advanced Science·Yaqin HuYanfei Liu
Aug 14, 2019·Chemical Society Reviews·Wenjing WangJun-Jie Zhu
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May 22, 2021·Analytical Chemistry·Liuchang MaMeng Liu
Jun 15, 2021·Chemical Science·Hong ZhangWei Jiang
Jul 15, 2021·Advanced Science·Shasha LuXiurong Yang
Jul 14, 2021·Chemical Reviews·Min LiQiang Xia
Apr 3, 2019·Analytical Chemistry·Hua Chai, Peng Miao
Jan 13, 2022·Analytical Chemistry·Chaoqun WangYi Lv

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