PKA and PKC activation induces opposite glial fibrillary acidic protein (GFAP) expression and morphology changes in a glioblastoma multiform cell line of clonal origin

Journal of Neuroscience Research
C ArcuriM Tardy

Abstract

Possible differentiation mechanisms were investigated in a glioblastoma multiform cell line (GL15) presenting an undifferentiated phenotype with weak glial fibrillary acidic protein (GFAP) and strong vimentin (VIM) expression. Serum-free conditions induced time-dependent increases of GFAP-mRNA and GFAP protein levels, associated with a process-bearing astrocytic morphology. Activation of protein kinase C (PKC) by tumor promoter phorbol 12-myrystate 13-acetate (PMA) induced a rapid morphological differentiation and a decrease in GFAP mRNA, whereas the GFAP level remained unchanged. Such parameters were shown to characterize a physiological differentiation stage in astroglial cultures. Treatment of process-bearing GL15 cells with dibutyryl cyclic AMP (dbcAMP), a protein kinase A (PKA) activator, induced a time-dependent decrease in the GFAP mRNA and GFAP protein levels and reverted morphological changes induced by serum-free conditions. Neither PMA nor dbcAMP influenced the VIM mRNA expression. In GL15 cells, PKC and PKA activation have opposite effects. Understanding the role of these kinases in malignant transformation and in the in vitro differentiation process is of both basic and clinical interest.

References

Mar 1, 1979·Journal of the National Cancer Institute·C M JacqueN Baumann
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G M WahlG R Stark
Jan 19, 1978·Acta Neuropathologica·B Lach, H Weinrauder
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·N S FoulkesP Sassone-Corsi
Oct 1, 1989·Journal of Neuroscience Research·S A MeyerK D McCarthy
Nov 29, 1986·Brain Research·P L MobleyE G Cruz
Jan 1, 1986·Acta Neuropathologica·U Roessmann, P Gambetti
May 7, 1971·Brain Research·L F EngB Gerstl
Dec 2, 1983·Neuroscience Letters·G J PilkingtonP L Lantos
Jan 1, 1981·Differentiation; Research in Biological Diversity·M OsbornK Bayreuther
Aug 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·V BocchiniM Tardy

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Citations

Nov 1, 2001·Brain Research. Molecular Brain Research·N YamamotoK Asai
Jul 16, 2002·Brain Research. Molecular Brain Research·Naoki YamamotoKiyofumi Asai
Jul 27, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·W L HuangS A Dunlop
Mar 27, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S L CostaM Tardy
Apr 17, 2012·Molecular Aspects of Medicine·Oana Benga, Vincent J Huber
Jul 21, 2009·Toxicon : Official Journal of the International Society on Toxinology·J P Silva-NetoS L Costa
Nov 18, 2000·American Journal of Physiology. Cell Physiology·E CastigliV Bocchini
Jan 27, 1999·Biochimica Et Biophysica Acta·G SorciR Donato

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