PMID: 8596704Feb 1, 1996Paper

PKC activators stimulate H+ conductance in chicken enterocytes

Pflügers Archiv : European journal of physiology
M L Calonge, A A Ilundáin

Abstract

Chicken enterocytes present a H+-conducting pathway involved in the recovery of intracellular pH (pHi) from an acid load. In the current study we have tested the effect of protein kinase C (PKC) activators on the rate of proton efflux through the H+-conducting pathway. The rate of proton efflux was increased by the addition of 1,2-dioctanoyl-rac-glycerol (DOG) or phorbol 12-myristate 13-acetate (PMA), but it was not affected by the addition of the inactive phorbol ester analogue, 4alpha-phorbol 12, 13-didecanoate. DOG stimulated the process in a dose-dependent manner with a half-maximal effect at 45 microM. Staurosporine and Zn2+ prevented the DOG-dependent increase in the rate of proton efflux. The rate of proton efflux was affected by the pH, and DOG shifted this relationship upward and to the right. These results suggest that the proton-conducting pathway is regulated by PKC.

References

Mar 1, 1992·Comparative Biochemistry and Physiology. Comparative Physiology·A Ilundáin
Jun 1, 1991·The American Journal of Physiology·E B ChangM C Rao
Dec 1, 1991·The American Journal of Physiology·J D Clark, L E Limbird
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A Nanda, S Grinstein
Jan 10, 1992·Biochimica Et Biophysica Acta·M L CalongeA Ilundáin
Dec 15, 1989·Biochemical and Biophysical Research Communications·V CasavolaH Murer
Dec 15, 1989·The Biochemical Journal·B DewaldM Baggiolini
Jun 1, 1984·The Biochemical Journal·M J Berridge
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P E Driedger, P M Blumberg
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Jan 26, 1995·Biochimica Et Biophysica Acta·M J PeralA A Ilundáin
Apr 1, 1995·The Journal of Physiology·M J Peral, A A Ilundáin
Jul 1, 1993·The American Journal of Physiology·G L LukacsS Grinstein

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Citations

Mar 29, 2003·Physiological Reviews·Thomas E Decoursey
Dec 31, 1997·The American Journal of Physiology·M GekleH Oberleithner
Aug 4, 1998·The American Journal of Physiology·C Eder

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