PKC-alpha shows variable patterns of translocation in response to different stimulatory agents

Acta Physiologica Scandinavica
C LiG L Wright

Abstract

Reports from numerous laboratories suggest that protein kinase C (PKC) translocation to substrate target sites may vary depending on cell type and experimental conditions. We have proposed that acutely variable targeting of PKC to different substrate sites could greatly expand the functional properties of individual isoforms in individual cell types (Li et al., 2001). Confocal microscopy and PKC alpha-enhanced green fluorescent protein (PKC alpha-EGFP) fusion protein expression were utilized to investigate the spatial and temporal pattern of PKC alpha translocation to different stimulating agents in A7r5 smooth muscle cells. Phorbol 12, 13 dibutyrate (PDBu 10(-8) M) caused a slow but irreversible relocation of the fusion protein from the cytosol to the plasmalemma. By comparison, thapsigargin (10(-5) M) and A23 187 (2 x 10(-5) M) induced a rapidly transient translocation to the cell membrane which was completed within 4 min. In contrast to these agents, angiotensin II (Ang II, 10(-6) M) caused only partial relocalization of cytosolic PKC alpha-EGFP to brightly fluorescing patches at the cell periphery. Localization at peripheral patches was completed within seconds and the fusion protein returned to the cytosol within 2 min. Th...Continue Reading

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Citations

Jun 24, 2010·Pflügers Archiv : European journal of physiology·HongLi PengMichael T Kirber
Jun 17, 2003·Molecular Biology of the Cell·Mario GimonaGerald Burgstaller
Aug 16, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Thomas J Eddinger
Apr 12, 2005·Vascular Pharmacology·K K MaddaliD K Bowles
Sep 10, 2004·Developmental Biology·D Page BaluchDavid G Capco
Mar 15, 2003·American Journal of Physiology. Cell Physiology·A C DykesG L Wright
Jan 4, 2020·Psychonomic Bulletin & Review·Roza G KamiloğluDisa A Sauter
Aug 2, 2019·The Journal of General Physiology·Vani S RavichandranMargaret V Westfall
May 11, 2004·World Journal of Gastroenterology : WJG·Tao MaWen-Xiu Yang
Jun 9, 2005·Gastroenterology·John F Di MariDon W Powell
Apr 20, 2006·Molecular and Cellular Endocrinology·Robert Rybczynski, Lawrence I Gilbert

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