PKCα and Netrin-1/UNC5B positive feedback control in relation with chemical therapy in bladder cancer.

European Review for Medical and Pharmacological Sciences
Jiao Liu, J Li

Abstract

To explore the cisplatin medical tolerance mechanism affecting bladder cancer cells. Bladder cancer cells were treated with protein kinase C α (PKCα) stimulation and inhibition agents. The small-interfering RNA (siRNA) inhibitory technique was used to differentiate and remove Netrin-1 from UNC5B. Cells treated by cisplatin were processed by the MIT method to estimate cell death and growth rate. The Western blot method was utilized to analyze PKCα, netrin-1, and UNC5B in bladder cancer cells, used in the relative control sample. Co-immunoprecipitation was employed to analyze PKCα, netrin-1, and UNC5B combination effects. PKCα high activity, netrin-1 high expression, and UNC5B with low expression can enhance bladder cancer cells cisplatin medical tolerance. PKCα low activity, netrin-1 low expression, and UNC5B with high expression can also enhance bladder cancer cells sensitivity to chemical therapeutic treatments. PKCα high activity with enhanced netrin-1 reduced UNC5B expression, and also enhanced netrin-1/UNC5B combination. It inhibits and/or deletes PKCα, Netrin-1 lower stream extracellular regulated protein kinases (ERK) signal, deletes UNC5B, PKCα, and lowers stream (ERK) signaling from activity. PKCα and netrin-1/UNC5B for...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Bo ZhanZhe Zhang
Zhonghua nan ke xue = National journal of andrology
Jiao Liu, Chuize Kong
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Chuize KongKun-feng Guo
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Jiao LiuChui-Ze Kong
© 2021 Meta ULC. All rights reserved