PKC isoforms in rat medullary thick ascending limb: selective activation of the delta-isoform by PGE2

The American Journal of Physiology
P C Aristimuño, D W Good

Abstract

In the medullary thick ascending limb (MTAL) of the rat kidney, prostaglandin E2 (PGE2) reverses inhibition of HCO3 absorption by arginine vasopressin (AVP). This effect of PGE2 is blocked by chelerythrine or staurosporine and mimicked by phorbol ester, suggesting a critical role for protein kinase C (PKC). The present study was designed to examine directly regulation of PKC isoforms by PGE2 in the inner stripe of the outer medulla and in microdissected MTALs. Immunoblots with isoform-specific anti-PKC antibodies detected alpha-, beta II-, delta-, epsilon-, and zeta-isoforms in both inner stripe and MTAL. The beta I- and gamma-isoforms were not detected. Translocation and activation of PKC were assessed by immunoblot analysis and by direct measurement of enzyme activity using an immune complex kinase assay. In inner stripe tissue incubated with 10(10) M AVP, PGE2 10(6) M for 20 min) induced translocation of PKC-delta from the cytosolic fraction to the membrane fraction. This translocation was associated with an 85% increase in PKC-delta activity in the membrane fraction and a 70% decrease in PKC-delta activity in the cytosolic fraction. PGE2 had no effect on the subcellular distribution or the activities of the other isoforms. ...Continue Reading

Citations

Feb 8, 2000·American Journal of Physiology. Renal Physiology·B M ChristensenS Nielsen
Mar 25, 2004·American Journal of Physiology. Renal Physiology·Stephanie RedlingVolker Vallon
Nov 23, 2007·American Journal of Physiology. Renal Physiology·Huda Ismail AbdullahNicholas R Ferreri
May 4, 2000·American Journal of Physiology. Cell Physiology·H J LiuN R Fererri
Mar 11, 2000·American Journal of Physiology. Renal Physiology·M ZeleninaA Aperia
Apr 25, 2000·The Journal of General Physiology·M S Awayda
Jan 30, 2004·American Journal of Physiology. Renal Physiology·Sebastian FrischeSusan M Wall
Jul 4, 2003·American Journal of Physiology. Cell Physiology·David W GoodBruns A Watts
Jun 18, 2010·American Journal of Physiology. Renal Physiology·David W GoodBruns A Watts
Mar 7, 2002·American Journal of Physiology. Renal Physiology·Hongyu ZhaoDavid M Cohen
Aug 9, 2003·American Journal of Physiology. Renal Physiology·Mohammad AsgharMustafa F Lokhandwala
Sep 13, 2002·American Journal of Physiology. Cell Physiology·Mouhamed S AwaydaAbderrahmane Bengrine
Sep 11, 2009·American Journal of Physiology. Renal Physiology·Jing YangPamela K Carmines
May 9, 2002·American Journal of Physiology. Renal Physiology·Bruns A Watts, David W Good
Jun 8, 2006·American Journal of Physiology. Renal Physiology·Bruns A WattsDavid W Good
Oct 13, 2000·American Journal of Physiology. Cell Physiology·D W GoodB A Watts

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