PKC isotypes in post-activated and fertilized mouse eggs: association with the meiotic spindle

Developmental Biology
D Page BaluchD G Capco

Abstract

Several isotypes of protein kinase C (PKC) have been reported to be expressed in mammalian eggs, but it is unknown whether these isotypes have a common function in the egg during or within the first few hours of fertilization. Here we show that the isotypes of PKC exhibit distinct patterns of enrichment immediately after mouse egg activation. PKCalpha and gamma accumulate in the egg cortex 25 min post-activation, while only PKCalpha accumulates at the contractile ring of the forming second polar body about 1.5 h post-activation. PKCzeta exhibits some unique features that resulted in it being the focus of more extensive analysis. PKCzeta is tightly associated with the meiotic spindle as determined by detergent extraction and is closely associated with alpha-tubulin as determined by FRET analysis in the metaphase II (MII) egg. In addition, after egg activation, PKCzeta remains associated with the spindle as it transits into anaphase II and later telophase II, becoming associated with the midzone microtubules. Antibodies to the active form of PKCzeta are enriched on the spindle poles and later in development on the midzone microtubules. Active PKCzeta also is enriched in both pronuclei in the 6-h post-fertilization and in the 14-h...Continue Reading

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Citations

Sep 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Hesam Dehghani, Ann C Hahnel
Mar 29, 2011·Cold Spring Harbor Perspectives in Biology·Takuya WakaiRafael A Fissore
Aug 7, 2013·Advances in Biological Regulation·Siti Nornadhirah AmdaniKevin Coward
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Jan 1, 2009·Molecular Reproduction and Development·Stephen A Stricker
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May 9, 2012·Journal of Cellular Physiology·Jose Raul Gonzalez-GarciaKarl Swann
Sep 25, 2009·Molecular Reproduction and Development·Madhavi KaliveDavid G Capco
Jul 24, 2015·Molecular Reproduction and Development·Wei MaMaria M Viveiros
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Feb 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Zhen-Yu ZhengQing-Yuan Sun
Oct 12, 2019·Experimental and Therapeutic Medicine·Yanchun LiuBingzhi Yu

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