Abstract
PKH26, a fluorescent cell label, and PKH95, a 125 I-radioactive cell label, are both potentially valuable endothelial cell labels because they bind irreversibly within cell membranes. These labels would be particularly well suited to tract transplanted endothelial cells in vivo. However, no previous studies documenting lack of transfer of the label to unlabeled endothelial cells, as well as the effect of the label on endothelial cell function, have been undertaken. The purpose of this study was to determine the optimal method of endothelial cell (EC) labeling with PKH26 and PKH95, whether significant to EC-to-EC transfer of the label occurs, the effects of these labels on EC proliferation and membrane function, and the feasibility of using these labels for long-term quantitative EC tracking in vivo. Canine ECs in confluent monolayers or in cell suspension were labeled by exposure to 1.0 or 5.0 microM PKH26 for 1, 3, or 5 min. Cell viability was determined by trypan blue exclusion. The percentage of cells labeled and their fluorescence intensity were determined in a fluorescent-activated cell sorter (FACS). Effect of the label on cell function was assessed by measuring EC proliferation rates as well as intercellular adhesion mol...Continue Reading
Citations
Jun 10, 2003·Plastic and Reconstructive Surgery·Bernd Rieck, Sabine Schlaak
Nov 9, 2007·Stem Cell Reviews·Li YanDaiming Fan
Dec 29, 2007·Immunological Investigations·Joseph J SkitzkiSharon S Evans
Feb 9, 2012·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Joshua Z PressElizabeth M Swisher
May 23, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Kanika ChawlaRobert L Sah
Mar 11, 2003·Tissue Engineering·M C KruytW J A Dhert
Nov 24, 2004·Circulation·John V Frangioni, Roger J Hajjar
May 30, 2007·Tissue Engineering·Kanika ChawlaRobert L Sah
Nov 28, 2013·BioMed Research International·Busadee PratumvinitMorayma Reyes
Mar 20, 2008·Plastic and Reconstructive Surgery·Kacey G MarraJ Peter Rubin
Jul 15, 1999·Cytometry·D J OhB O Palsson
Dec 1, 2007·Transplantation·Jui-Yang LaiGing-Ho Hsiue
Oct 19, 2000·Antimicrobial Agents and Chemotherapy·C Di GiorgioP Timon-David
Mar 13, 2008·PloS One·Nicholas IeronimakisMorayma Reyes
Feb 26, 2000·In Vitro Cellular & Developmental Biology. Animal·G M LeeB O Palsson
Oct 29, 2000·Cell Adhesion and Communication·M H JuulT Sanner
Jan 28, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·W de-SouzaM Vieira
Dec 29, 2007·Immunological Investigations·Nadir AskenasyDaniel L Farkas
Jan 5, 2002·In Vitro Cellular & Developmental Biology. Animal·C RousselleX Ronot
Aug 24, 1999·The Journal of Surgical Research·D FoxH P Greisler
May 13, 2021·Seminars in Cancer Biology·Sukanya BasuDean G Tang