PKS5, a SNF1-related kinase, interacts with and phosphorylates NPR1, and modulates expression of WRKY38 and WRKY62

Journal of Genetics and Genomics = Yi Chuan Xue Bao
Changgen XieYan Guo

Abstract

NPR1 (Nonexpressor of Pathogenesis-Related gene 1) is a major co-activator of plant defense. Phosphorylations of NPR1 play important roles in fine-tuning its activity, however a kinase corresponding to such modification remains uncharacterized. Here, we report that NPR1 interacts with PKS5 (SOS2-like Protein Kinase 5). The AKR (AnKyrin Repeats) motif of NPR1 is required for this interaction. PKS5 phosphorylates NPR1 at the C-terminal region. Expression of PKS5 is induced quickly by Pseudomonas syringae pv. tomato DC3000. Expression level of two NPR1 target genes, WRKY38 and WRKY62, is reduced and/or delayed in pks5 mutants. Moreover, the expression of WRKY38 and WRKY62 displays a similar pattern in npr1-1pks5-1 double mutant comparing to that in npr1-1. Our results suggest that PKS5 functions at the upstream of NPR1 and might mediate expression of WRKY38 and WRKY62 possibly by interacting with and phosphorylating NPR1.

References

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Citations

May 21, 2013·Trends in Plant Science·Karolina M Pajerowska-MukhtarM Shahid Mukhtar
Jun 28, 2011·Current Opinion in Plant Biology·Guillaume TenaJen Sheen
Dec 19, 2012·Biochemical and Biophysical Research Communications·Xiaona ZhouChang Gen Xie
Nov 26, 2013·Biochemical and Biophysical Research Communications·Feifei YuanChang Gen Xie
May 21, 2014·Current Opinion in Plant Biology·Shunping Yan, Xinnian Dong
Jul 5, 2016·Frontiers in Plant Science·Ujjal J PhukanRakesh K Shukla
Dec 16, 2017·MSphere·Louisi Souza de OliveiraFabiano L Thompson
Oct 12, 2018·Molecular Plant-microbe Interactions : MPMI·Kerry F PedleyMichelle A Graham
May 31, 2019·The Plant Journal : for Cell and Molecular Biology·Feng Y CaoDarrell Desveaux
Jan 14, 2020·Plant, Cell & Environment·Prasanna KankanalaKirankumar S Mysore
Feb 5, 2014·Plant Signaling & Behavior·Madhunita Bakshi, Ralf Oelmüller
May 19, 2019·International Journal of Molecular Sciences·Yanlin MaYi Yang
Jan 27, 2021·Plant, Cell & Environment·Jian ChenFengquan Liu
May 28, 2019·Plant Science : an International Journal of Experimental Plant Biology·Matthew J MarcecKiwamu Tanaka

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