Placenta-derived chymotrypsin-like protease (CLP) disturbs endothelial junctional structure in preeclampsia.

Reproductive Sciences
Yang GuYuping Wang

Abstract

Placenta-derived chymotrypsin-like protease may contribute to endothelial activation in preeclampsia. In this study, we determined if placenta-derived chymotrypsin-like protease could disturb endothelial junctional integrity to promote endothelial permeability in preeclampsia. Confluent endothelial cells were cocultured with placental trophoblasts or treated with preeclampsia placenta-conditioned medium. Endothelial junction protein vascular endothelial cadherin expression and distribution were examined by fluorescent staining of endothelial cells with or without depletion of chymotrypsin. The association of endothelial cell junction protein complex VE-cadherin/beta-catenin/p120 was examined by a combined immuno-precipitation and immuno-blotting assay. Our results showed that endothelial cells cocultured with preeclampsia trophoblasts or exposed to preeclampsia placental conditioned medium exhibited a discontinuous distribution and reduced expression of vascular endothelial cadherin at cell contact regions. Vascular endothelial cadherin and p120 were expressed in control endothelial cells, but reduced or lost in endothelial cells exposed to preeclampsia placental conditioned medium, suggesting that the junctional protein comple...Continue Reading

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Citations

Feb 22, 2014·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Kuan YangRonald B Walter
Nov 30, 2011·Drug Discovery Today. Disease Models·Hongyan Dai, Ronald J Korthuis
Jul 13, 2013·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·James S BetoniGerard Tromp
Jan 20, 2018·Circulation Research·Louis J Dell'ItaliaCarlos M Ferrario
Feb 9, 2021·Frontiers in Medicine·Elisavet StavropoulouEugenia Bezirtzoglou

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