Placenta-specific drug delivery by trophoblast-targeted nanoparticles in mice

Theranostics
Baozhen ZhangXiujun Fan

Abstract

Rationale: The availability of therapeutics to treat pregnancy complications is severely lacking, mainly due to the risk of harm to the fetus. In placental malaria, Plasmodium falciparum-infected erythrocytes (IEs) accumulate in the placenta by adhering to chondroitin sulfate A (CSA) on the surfaces of trophoblasts. Based on this principle, we have developed a method for targeted delivery of payloads to the placenta using a synthetic placental CSA-binding peptide (plCSA-BP) derived from VAR2CSA, a CSA-binding protein expressed on IEs. Methods: A biotinylated plCSA-BP was used to examine the specificity of plCSA-BP binding to mouse and human placental tissue in tissue sections in vitro. Different nanoparticles, including plCSA-BP-conjugated nanoparticles loaded with indocyanine green (plCSA-INPs) or methotrexate (plCSA-MNPs), were administered intravenously to pregnant mice to test their efficiency at drug delivery to the placenta in vivo. The tissue distribution and localization of the plCSA-INPs were monitored in live animals using an IVIS imaging system. The effect of plCSA-MNPs on fetal and placental development and pregnancy outcome were examined using a small-animal high-frequency ultrasound (HFUS) imaging system, and the ...Continue Reading

Citations

Jan 22, 2020·Journal of Receptor and Signal Transduction Research·Yonglan ZhangWei Wang
Feb 19, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wei PengPhilip N Baker
Sep 27, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gang WangXin Ni
Apr 25, 2019·Current Medical Science·Jian-Li WuLing Feng
May 27, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·N'Dea S Irvin-ChoyEmily S Day
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Lei LiShan Wang
Dec 19, 2019·American Journal of Physiology. Cell Physiology·Zhilong ChenQing Yang
Jul 28, 2019·International Journal of Molecular Sciences·Baozhen ZhangXiujun Fan
Jul 1, 2020·Biomolecules·Manoj Kumar JenaNihar Ranjan Nayak
Nov 24, 2020·Clinical and Experimental Reproductive Medicine·Ruchita ShandilyaPradyumna Kumar Mishra
Dec 7, 2020·Human Reproduction Update·Natasha PritchardNatalie Hannan
Feb 3, 2021·The FEBS Journal·Mingyu HuChao Tong
Oct 2, 2020·Advanced Drug Delivery Reviews·Katherine M NelsonEmily S Day
Mar 27, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Shahla ShojaeiHagar I Labouta
Apr 4, 2021·Antioxidants·Xiang-Qun Hu, Lubo Zhang
Feb 13, 2019·Placenta·Carole-Anne WhighamTu'uhevaha J Kaitu'u-Lino
Aug 30, 2021·Advanced Drug Delivery Reviews·Anjali SharmaRangaramanujam M Kannan

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Methods Mentioned

BETA
transmission electron microscopy
fluorescence spectroscopy
fluorescence
confocal microscopy

Software Mentioned

Living Image
ImageJ
Vevo 2100
GraphPad
GraphPad Prism

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