Placental growth factor enhances angiogenesis in human intestinal microvascular endothelial cells via PI3K/Akt pathway: Potential implications of inflammation bowel disease

Biochemical and Biophysical Research Communications
Yi ZhouShuncai Zhang

Abstract

Angiogenesis plays a major role in the pathogenesis of inflammatory bowel disease (IBD). Placental growth factor (PlGF) is a specific regulator of pathological angiogenesis and is upregulated in the sera of IBD patients. Therefore, the role of PlGF in IBD angiogenesis was investigated here using HIMECs. The expression of PlGF and its receptors in human intestinal microvascular endothelial cells (HIMECs) and inflamed mucosa of IBD patients were examined using quantitative PCR and western blot analysis and the role of PlGF in IBD HIMECs was further explored using small interfering RNA (siRNA). The induction of pro-inflammatory cytokine by PlGF in HIMECs was confirmed by ELISA. The capacity of PlGF to induce angiogenesis in HIMECs was tested through proliferation, cell-migration, matrigel tubule-formation assays and its underlying signaling pathway were explored by western blot analysis of ERK1/2 and PI3K/Akt phosphorylation. mRNA and protein expression of PlGF and its receptor NRP-1 were significantly increased in IBD HIMECs. Inflamed mucosa of IBD patients also displayed higher expression of PIGF. The production of IL-6 and TNF-α in culture supernatant of HIMECs treated with exogenous recombinant human PlGF-1 (rhPlGF-1) were inc...Continue Reading

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Citations

Jan 31, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Jia-Yuan ZhuangZe-Hao Zhuang
Jun 21, 2019·International Journal of Molecular Sciences·Loredana AlboniciRoberto Bei
Mar 17, 2020·Frontiers in Oncology·Yolla HaibeAli Shamseddine
Mar 18, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·ChunMei LiuYingyu Jin
Sep 27, 2018·World Journal of Clinical Oncology·Candice CarolaBenoist Chibaudel
Apr 20, 2018·International Journal of Molecular Sciences·Yoshiro ItataniYoshiharu Sakai

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