Placental growth factor in beta cells plays an essential role in gestational beta-cell growth

BMJ Open Diabetes Research & Care
Weixia YangXiangwei Xiao

Abstract

Pancreatic beta cells proliferate in response to metabolic requirements during pregnancy, while failure of this response may cause gestational diabetes. A member of the vascular endothelial growth factor family, placental growth factor (PlGF), typically plays a role in metabolic disorder and pathological circumstance. The expression and function of PlGF in the endocrine pancreas have not been reported and are addressed in the current study. PlGF levels in beta cells were determined by immunostaining or ELISA in purified beta cells in non-pregnant and pregnant adult mice. An adeno-associated virus (AAV) serotype 8 carrying a shRNA for PlGF under the control of a rat insulin promoter (AAV-rat insulin promoter (RIP)-short hairpin small interfering RNA for PlGF (shPlGF)) was prepared and infused into mouse pancreas through the pancreatic duct to specifically knock down PlGF in beta cells, and its effects on beta-cell growth were determined by beta-cell proliferation, beta-cell mass and insulin release. A macrophage-depleting reagent, clodronate, was coapplied into AAV-treated mice to study crosstalk between beta cells and macrophages. PlGF is exclusively produced by beta cells in the adult mouse pancreas. Moreover, PlGF expression ...Continue Reading

Citations

Mar 12, 2021·The Journal of Physiology·Esteban Roberto Salazar-Petres, Amanda Nancy Sferruzzi-Perri
Apr 7, 2021·Experimental & Molecular Medicine·Qun LiuXiangwei Xiao

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Methods Mentioned

BETA
RIP
transgenic
reverse transcription PCR
Assay
fluorescence-activated cell sorting
ELISA
fluorescence-activated

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