Placental lipase expression in pregnancies complicated by preeclampsia: a case-control study

Reproductive Biology and Endocrinology : RB&E
Helen L BarrettMarloes Dekker Nitert

Abstract

Preeclampsia (PE) is associated with maternal and neonatal morbidity and mortality. In PE, the physiological hyperlipidaemia of pregnancy is exaggerated. The purpose of this study was to examine the expression of adipose triglyceride lipase (ATGL), hormone sensitive lipase (HSL), lipoprotein lipase (LPL) and endothelial lipase (EL) in pregnancies complicated by PE. Placentae were collected from 16 women with PE and 20 women with uncomplicated pregnancies matched for maternal prepregnancy BMI and gestational age of delivery. Gene and protein expression of the placental lipases were measured by Q-PCR and Western blot. DNA methylation of the promoter of LPL was assessed by bisulfite sequencing. Lipase localisation and activity were analysed. Gene expression of all lipases was significantly reduced, as was HSL protein level in women with PE. All lipases were localised to trophoblasts and endothelial cells in PE and control placentae. There was no difference in methylation of the LPL promoter between PE and control placentae. Lipase activity was not altered in placentae from women with PE. These results suggest that the decreased placental lipase gene but not protein expression or lipase activity, which is associated with late-onset...Continue Reading

References

Jan 1, 1985·Acta Obstetricia Et Gynecologica Scandinavica·Y Biale
Sep 20, 2002·Journal of Atherosclerosis and Thrombosis·Kazuhiro OguraGen Yoshino
Apr 5, 2005·Clinical Obstetrics and Gynecology·Mariëlle G van Pampus, Jan G Aarnoudse
Jul 29, 2005·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Gokhan BayhanZeki Akkus
Mar 24, 2006·BJOG : an International Journal of Obstetrics and Gynaecology·J G RayC M Bell
Apr 15, 2006·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Saeideh ZiaeiAnoshirvan Kazemnejad
Feb 15, 2007·American Journal of Physiology. Endocrinology and Metabolism·Anne Liese Magnusson-OlssonTheresa L Powell
Mar 16, 2007·The Journal of Clinical Endocrinology and Metabolism·M GausterC Wadsack
Oct 27, 2007·Journal of the Chinese Medical Association : JCMA·Usha AdigaNandini Mangalore
Dec 25, 2008·American Journal of Physiology. Endocrinology and Metabolism·Thomas J AlstedBente Kiens
Jul 2, 2009·The Australian & New Zealand Journal of Obstetrics & Gynaecology·Sandra A LoweUNKNOWN Society of Obstetric Medicine of Australia and New Zealand
Feb 20, 2010·Obstetrics and Gynecology·Elisabeth B MagnussenPål R Romundstad
Jan 5, 2011·International Journal of Pediatrics·Patricia Y L ChanEileen D M Gallery
May 6, 2011·The American Journal of Clinical Nutrition·Gernot DesoyeChristian Wadsack
Oct 25, 2011·Obstetrical & Gynecological Survey·Dahlia Raymond, Erika Peterson
Dec 14, 2011·Applied Immunohistochemistry & Molecular Morphology : AIMM·Stephen C HelpsRobert Vink
Mar 28, 2012·Current Opinion in Clinical Nutrition and Metabolic Care·Alfonso Gil-SánchezElvira Larqué
Jun 1, 2012·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·C Mayer, K S Joseph
Jul 19, 2012·Journal of Clinical Ultrasound : JCU·Gokhan GoynumerCihan Karadag
Aug 15, 2014·PloS One·Helen L BarrettMarloes Dekker Nitert

❮ Previous
Next ❯

Citations

May 10, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Valérie Gagné-OuelletLuigi Bouchard
Jun 24, 2020·Cancer Gene Therapy·Chang HongJie Lin
Aug 25, 2020·Journal of Hypertension·Amrita A KhaireSadhana R Joshi
May 1, 2021·Nutrients·Maciej ZiętekMałgorzata Szczuko

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Infrared Imaging
SGA
biopsies

Software Mentioned

NIH
Prism
Methprimer
GraphPad
ImageJ

Related Concepts

Related Feeds

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Related Papers

International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity
M KolehmainenM I J Uusitupa
American Journal of Obstetrics and Gynecology
Andrea G EdlowMichal A Elovitz
Journal of the Society for Gynecologic Investigation
T LamlP Wagenbichler
© 2021 Meta ULC. All rights reserved