Placental Metabolomics for Assessment of Sex-specific Differences in Fetal Development During Normal Gestation.

Scientific Reports
Michelle SaoiPhilip Britz-McKibbin

Abstract

The placenta is a metabolically active interfacial organ that plays crucial roles in fetal nutrient delivery, gas exchange and waste removal reflecting dynamic maternal and fetal interactions during gestation. There is growing evidence that the sex of the placenta influences fetal responses to external stimuli in utero, such as changes in maternal nutrition and exposure to environmental stressors. However, the exact biochemical mechanisms associated with sex-specific metabolic adaptations during pregnancy and its link to placental function and fetal development remain poorly understood. Herein, multisegment injection-capillary electrophoresis-mass spectrometry is used as a high throughput metabolomics platform to characterize lyophilized placental tissue (~2 mg dried weight) from C57BL/6J mice fed a standardized diet. Over 130 authentic metabolites were consistently measured from placental extracts when using a nontargeted metabolomics workflow with stringent quality control and robust batch correction. Our work revealed distinct metabolic phenotype differences that exist between male (n = 14) and female (n = 14) placentae collected at embryonic day E18.5. Intracellular metabolites associated with fatty acid oxidation and purin...Continue Reading

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Citations

Sep 22, 2020·American Journal of Physiology. Endocrinology and Metabolism·Serene JosephMaureen Keller-Wood
Sep 10, 2020·Electrophoresis·Wei Zhang, Rawi Ramautar
Mar 24, 2021·Analytical and Bioanalytical Chemistry·Ziting Gao, Wenwan Zhong
Aug 1, 2021·Placenta·Monica StrawnSusanta K Behura
Oct 27, 2021·Reproductive Sciences·Julian K Christians
Oct 6, 2021·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jonathan C BehlenNatalie M Johnson
Nov 10, 2021·Journal of Clinical Laboratory Analysis·Yuqi YangBin Yu

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Methods Mentioned

BETA
PCA
NMR
electrophoresis

Software Mentioned

Excel
Agilent MassHunter Database Library
MetFragWeb
SPSS
Agilent MassHunter Qualitative Analysis
Metaboanalyst
BatchCorrMetabolomics
R package

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