Abstract
Premature rupture of membranes (PROM) is a common pregnancy complication that frequently results in maternal and perinatal morbidity. The present methods for diagnosing PROM do not satisfy clinical requirements. The present study aimed to examine the proteome profile of amniotic fluid (AF) and maternal plasma, screen unique proteins in AF, and evaluate their diagnostic value for diagnosing PROM. The proteome profiles of AF and maternal plasma were examined via liquid chromatography coupled with tandem mass spectrometry‑based proteomic techniques. The protein expression levels of diagnostic candidates in AF, maternal plasma and vaginal fluid were determined by ELISA analysis and Magnetic Luminex® screening assays. The diagnostic value of potential biomarkers was evaluated using receiver operating characteristic curves. A lateral flow assay was developed based on colloidal gold immunochromatography technology. The present study identified 540 unique proteins in AF, 12 of which were chosen for further detection. The present results demonstrated that expression levels of pulmonary surfactant‑associated protein B, BPI fold‑containing family A member 1, zymogen granule protein 16 homolog B, EGF‑containing fibulin‑like extracellular m...Continue Reading
References
Apr 1, 1989·American Journal of Perinatology·P Bischof
Dec 1, 1982·Journal of Medical Genetics·W W Chen
Mar 1, 1981·British Journal of Obstetrics and Gynaecology·O TydénB A Nilsson
Feb 1, 1994·Early Human Development·K ChatzakisT Chard
Dec 12, 2001·Blood·J RachmilewitzM L Tykocinski
Sep 7, 2002·Journal of Immunological Methods·N GrebenchtchikovTh J Benraad
Apr 8, 2009·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Seung Mi LeeBo Hyun Yoon
Jul 8, 2009·Journal of the Chinese Medical Association : JCMA·Xing-Long TongYan-Ping Xu
Jun 24, 2010·Journal of Obstetrics and Gynaecology Canada : JOGC = Journal D'obstétrique Et Gynécologie Du Canada : JOGC·Amira El-Messidi, Alan Cameron
Apr 22, 2011·Clinical Obstetrics and Gynecology·Thaddeus P Waters, Brian Mercer
Jun 2, 2011·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Federico G Mariona, Lluis Cabero
Jun 10, 2011·Proteomics. Clinical Applications·Tao WangHuaizhong Hu
Oct 26, 2012·Journal of Perinatal Medicine·Tao WangHuaizhong Hu
Apr 10, 2014·Archives of Gynecology and Obstetrics·Ibrahim A AbdelazimAmr F Abdulkareem
Jun 4, 2014·The Journal of Obstetrics and Gynaecology Research·De-ku LiangXiao-yan Jia
Dec 30, 2014·Cytokine & Growth Factor Reviews·V C RussoG A Werther
Dec 1, 2017·OncoTargets and Therapy·Paolo Baldo, Sara Cecco