Placental trophoblast debris mediated feto-maternal signalling via small RNA delivery: implications for preeclampsia

Scientific Reports
Jia WeiL Chamley

Abstract

To profile the small RNA cargo carried by trophoblast debris derived from the placenta during normal and preeclamptic pregnancies and to determine whether trophoblast debris can deliver its small RNAs to endothelial cells with functional consequences. We confirmed that trophoblast debris can deliver its small RNAs contents to recipient endothelial cells during the co-culture. Next generation sequencing was employed to profile the small RNA contents in both normotensive and preeclamptic trophoblast debris. We identified 1278 mature miRNAs and 2646 non-miRNA small RNA fragments contained. Differential expression analysis identified 16 miRNAs (including miR-145), 5 tRNA fragments from 3 different tRNAs, 13 snRNA fragments and 85 rRNA fragments that were present in different levels between preeclamptic and normotensive trophoblast debris. We loaded a miR-145 mimic into normotensive trophoblast debris via transfection of placental explants from which the debris was derived and found the miR-145 loaded debris induced transcriptomic changes in endothelial cells similar to those induced by preeclamptic trophoblast debris. Trophoblast debris deported into maternal circulation can deliver its small RNA contents to maternal cells thereby ...Continue Reading

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Citations

Apr 1, 2018·Immunology and Cell Biology·Mancy TongLawrence W Chamley
Apr 16, 2019·Frontiers in Physiology·Kenta Hyeon Tae ChoMhoyra Fraser
Jan 9, 2019·Frontiers in Physiology·Rafael Tomoya MichitaJosé Artur Bogo Chies
Dec 1, 2020·American Journal of Reproductive Immunology : AJRI·Rodolfo R FavaroUdo R Markert

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Methods Mentioned

BETA
confocal microscopy
confocal
transfection
electrophoresis
cDNA
Assay
PCR

Software Mentioned

DESEq
NZGL
EdgeR
GraphPad Prism
iSRAP

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