Plagiochin E, a botanic-derived phenolic compound, reverses fungal resistance to fluconazole relating to the efflux pump

Journal of Applied Microbiology
X-L GuoH-X Lou

Abstract

In this study, we investigated the effect of plagiochin E (PLE), a botanic-derived phenolic natural product, on reversal of fungal resistance to fluconazole (FLC) in vitro and the related mechanism. A synergistic action of PLE and FLC was observed in the FLC-resistant Candida albicans strains and was evaluated using the fractional inhibited concentration index. The effect of PLE on FLC intracellular uptake was investigated in FLC-resistant C. albicans cells by liquid chromatography-tandem mass spectrometry, and the effect on efflux drug pump was assessed by measuring the efflux of Rhodamine 123 (Rh123). PLE significantly inhibited the efflux, but not the absorption, of Rh123 in FLC-resistant strains in phosphate-buffered saline with 5% glucose. Overexpression of the multidrug-resistance gene CDR1 in FLC-resistant C. albicans isolates was detected, and the introduction of PLE to the cells showed a significant reduction of the CDR1 expression in those FLC-resistant isolates. These findings indicate that PLE could reverse the fungal resistant to FLC by inhibiting the efflux of FLC from C. albicans, and this effect may be related to the efflux pump. These results indicate that the combination of PLE and FLC may provide an approach ...Continue Reading

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Citations

Feb 26, 2010·Natural Product Research·Chun-Feng XieHong-Xiang Lou
Aug 5, 2009·Biological & Pharmaceutical Bulletin·Aixia ChengHongxiang Lou
Jul 25, 2014·PloS One·Wei LiuYuan Ying Jiang
Jan 1, 2009·Beilstein Journal of Organic Chemistry·Andreas SpeicherJürgen Kolz
Jun 2, 2012·Phytochemistry·Yoshinori AsakawaFumihiro Nagashima
Oct 22, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Xia LiHong-Xiang Lou
Dec 17, 2009·Journal of Applied Microbiology·L M SunH X Lou
Nov 29, 2016·Expert Opinion on Therapeutic Targets·Clarissa WillersJosias Hendrik Hamman
Jun 8, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Saikat DewanjeeMuhammad Zia-Ul-Haq
Mar 26, 2009·Chemistry & Biodiversity·Chun-Feng Xie, Hong-Xiang Lou
Mar 19, 2020·Current Pharmaceutical Design·Kleber Santiago SilvaMaristela Pereira
May 30, 2009·Chemistry & Biodiversity·Xiao-Ning WangHong-Xiang Lou
Aug 16, 2013·Beilstein Journal of Organic Chemistry·Willmen YoungsayeBenito Munoz
Sep 21, 2018·Frontiers in Cellular and Infection Microbiology·Yiman LiShujuan Sun
Aug 18, 2020·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Samapika Nandy, Abhijit Dey
Oct 12, 2017·Journal of Natural Products·Yoshinori Asakawa, Agnieszka Ludwiczuk
Aug 23, 2021·Chinese Journal of Natural Medicines·Fu-Juan SunMing-Hua Yang

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