Planar and Cell Aggregate-Like Assemblies Consisting of Microreactors and HepG2 Cells

ACS Omega
Yan ZhangBrigitte Städler

Abstract

The assembly of microreactors has made considerable progress toward the fabrication of artificial cells. However, their characterization remains largely limited to buffer solution-based assays in the absence of their natural role model-the biological cells. Herein, the combination of microreactors with HepG2 cells either in planar cell cultures or in the form of cell aggregates is reported. Alginate (Alg)-based microreactors loaded with catalase are assembled by droplet microfluidics, and their activity is confirmed. The acceptance of polymer-coated ∼40 μm Alg particles by proliferating HepG2 cells is depending on the terminating polymer layer. When these functional microreactors are cocultured with HepG2 cells, they can be employed for detoxification, that is, hydrogen peroxide removal, and by doing so, they assist the cells to survive. This report is among the first successful combination of microreactors with biological cells, that is, HepG2 cells, contributing to the fundamental understanding of integrating synthetic and biological partners toward the maturation of this semisynthetic concept for biomedical applications.

References

Aug 13, 2005·Macromolecular Bioscience·Rohit SrivastavaMichael J McShane
Aug 19, 2007·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Senay Hamarat Baysal
Jan 20, 2010·Journal of Cellular and Molecular Medicine·Verena M C QuentDietmar W Hutmacher
Aug 26, 2010·Angewandte Chemie·Stijn F M van DongenJan C M van Hest
Jan 21, 2011·Chemical Communications : Chem Comm·Zhikang FuMadhavan Nallani
Mar 3, 2011·Chemistry : a European Journal·Pascal TannerCornelia G Palivan
May 26, 2011·Accounts of Chemical Research·Pascal TannerWolfgang Meier
Aug 16, 2011·Journal of the American Chemical Society·Shin-Hyun KimDavid A Weitz
Oct 14, 2011·Nano Letters·Rona ChandrawatiFrank Caruso
Nov 30, 2011·Progress in Polymer Science·Kuen Yong Lee, David J Mooney
Jul 27, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Rona Chandrawati, Frank Caruso
Oct 18, 2012·Chemical Society Reviews·Maïté MarguetSébastien Lecommandoux
Oct 31, 2012·Colloids and Surfaces. B, Biointerfaces·T D Dang, S W Joo
Nov 20, 2012·Electrophoresis·Yung-Sheng LinChen-Ling Hsieh
Jan 2, 2013·Journal of Colloid and Interface Science·Martin UllrichFrantišek Štěpánek
Jan 22, 2013·Biomacromolecules·Clara R CorreiaJoão F Mano
Mar 7, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Jaroslav HanušFrantišek Stěpánek
Mar 22, 2013·ACS Applied Materials & Interfaces·Martin E LyngeBrigitte Städler
May 8, 2013·Nano Letters·Pascal TannerCornelia G Palivan
Nov 21, 2013·Angewandte Chemie·Ruud J R W PetersSébastien Lecommandoux
Mar 14, 2014·Nature·Eric K SackmannDavid J Beebe
Apr 4, 2014·ACS Applied Materials & Interfaces·Feng-Peng ChangChung-Yuan Mou
Jun 27, 2014·ACS Applied Materials & Interfaces·Leticia Hosta-RigauBrigitte Städler
Jun 10, 2015·Soft Matter·Philipp SchattlingBrigitte Städler
Jul 23, 2015·Macromolecular Bioscience·Linas MazutisDavid A Weitz
Jul 28, 2016·Scientific Reports·Minggan LiDae Kun Hwang
Nov 20, 2016·Nanoscale·Ana Armada-MoreiraBrigitte Städler
Dec 3, 2016·Advanced Healthcare Materials·Yan ZhangBrigitte Städler
Dec 23, 2016·Advanced Healthcare Materials·María Godoy-GallardoLeticia Hosta-Rigau
Jun 29, 2017·ACS Nano·Tatiana TrantidouOscar Ces
Mar 26, 2013·Materials·Jinchen Sun, Huaping Tan
May 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Winna SitiBo Liedberg

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Citations

Sep 2, 2020·Advanced Biosystems·Adam Armada-MoreiraSandra H Vaz
Nov 28, 2018·Advanced Science·Tomoki Nishimura, Kazunari Akiyoshi
Sep 20, 2019·Biomaterials Science·Maria Jose York-DuranLeticia Hosta-Rigau
Dec 20, 2017·Biomaterials Science·Chuntao ZhuBrigitte Städler
Nov 19, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Xiaomin QianBrigitte Städler
Aug 17, 2018·ACS Applied Materials & Interfaces·Fabian ItelBrigitte Städler
Sep 20, 2018·Biomacromolecules·Chuntao ZhuBrigitte Städler
Jan 4, 2019·ACS Applied Materials & Interfaces·Maria Godoy-GallardoLeticia Hosta-Rigau
Aug 26, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Masataka Iwasaki, Makoto Yoshimoto

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Methods Mentioned

BETA
scanning electron microscopy
Fluorescent microscopy

Software Mentioned

ImageJ

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