Planar cell polarity protein Dishevelled 3 (Dvl3) regulates ectoplasmic specialization (ES) dynamics in the testis through changes in cytoskeletal organization

Cell Death & Disease
Linxi LiC Yan Cheng

Abstract

In the mammalian testes, such as in rats, the directional alignment of polarized elongating/elongated spermatids, in particular step 17-19 spermatids, across the plane of seminiferous epithelium resembles planar cell polarity (PCP) found in hair cells of the cochlea. It is obvious that spermatid PCP is necessary to support the simultaneous development of maximal number of elongating/elongated spermatids to sustain the daily production of > 50 million sperm per adult rat. Studies have shown that the testis indeed expresses multiple PCP proteins necessary to support spermatid PCP. Herein, using physiological and biochemical assays, and morphological analysis, and with the technique of RNA interference (RNAi) to knockdown PCP protein Dishevelled (Dvl) 1 (Dvl1), Dvl2, Dvl3, or Dvl1/2/3, Dvl proteins, in particular Dvl3, it was shown that Dvl3 played a crucial role of support Sertoli cell tight junction (TJ)-permeability barrier function through changes in the organization of actin- and microtubule (MT)-based cytoskeletons. More important, an in vivo knockdown of Dvl1/2/3 in the testis, defects of spermatid polarity were remarkably noted across the seminiferous epithelium, concomitant with defects of spermatid adhesion and spermatid...Continue Reading

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Citations

Mar 25, 2020·Critical Reviews in Biochemistry and Molecular Biology·Linxi LiC Yan Cheng
Jul 11, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Lingling WangC Yan Cheng
Jan 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shiwen LiuC Yan Cheng
Jun 2, 2021·Seminars in Cell & Developmental Biology·Lingling WangC Yan Cheng

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Methods Mentioned

BETA
electron microscopy
transfection
PCR
protein assay
Fluorescence

Software Mentioned

Excel
GraphPad
Nikon NIS Elements AR
Adobe Photoshop
ImageJ
ImageQuant
Multi
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GraphPad Prism

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