Planktonic cell yield is linked to biofilm development

Canadian Journal of Microbiology
E BesterG M Wolfaardt

Abstract

We report on the ability of surface-associated microbes to produce and release single planktonic cells to the bulk liquid as early as 6 h after attachment, with pure culture and mixed-species biofilms yielding up to approximately 1 x 10(6) cells/cm(2) of attachment area per hour to the effluent after 24 h. Planktonic cell production typically increased as the biofilm developed and levelled off after the biofilm reached steady-state dimensions. Microscopic observations of continuous-flow cultured biofilms revealed independent cell movement within the biofilm microenvironment compared with flow-dependent movement of mostly single cells in the bulk-liquid phase. These results indicate that the prevailing concept of detachment occurring only after the biofilm has matured is incomplete. Instead, we show that biofilms yield cells to the environment soon after initial surface contact; the extent of this yield is dependent on biofilm development, which in turn is influenced by environmental parameters such as bulk-liquid flow rates and nutrient availability. The observation that biofilms yield significant numbers of cells throughout development should lead to a greater understanding of pathogen dissemination, biofouling of products or ...Continue Reading

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Citations

Dec 2, 2011·Bulletin of Mathematical Biology·Alma Mašić, Hermann J Eberl
Sep 18, 2013·Biofouling·Evan RonanGideon M Wolfaardt
Jul 20, 2010·Applied and Environmental Microbiology·Otini KroukampGideon M Wolfaardt
Dec 22, 2009·Applied and Environmental Microbiology·Elanna BesterSteven N Liss
Sep 25, 2012·Applied and Environmental Microbiology·Jessica R SheldonKam Tin Leung
Oct 23, 2012·Applied and Environmental Microbiology·Alexandru DumitracheLee R Lynd
Dec 18, 2010·Canadian Journal of Microbiology·Jerry ChaoMichael T Arts
Nov 10, 2013·International Journal of Molecular Sciences·Elanna BesterJesse Greener
Aug 26, 2014·Applied Microbiology and Biotechnology·Peter J SchnurrD Grant Allen
Mar 1, 2014·Microbial Ecology·Mahtab GhadakpourGideon M Wolfaardt
Apr 26, 2016·Ultrasonics·Evan RonanRaffi Karshafian
Jul 15, 2015·Canadian Journal of Microbiology·Andrew J SousaGideon Wolfaardt
Jul 22, 2015·PloS One·Blessing O EmereniniHermann J Eberl
Apr 21, 2017·Journal of Biological Dynamics·Qasim Ali, Lindi M Wahl

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