Plant 24-nt reproductive phasiRNAs from intramolecular duplex mRNAs in diverse monocots

Genome Research
Atul KakranaBlake C Meyers

Abstract

In grasses, two pathways that generate diverse and numerous 21-nt (premeiotic) and 24-nt (meiotic) phased siRNAs are highly enriched in anthers, the male reproductive organs. These "phasiRNAs" are analogous to mammalian piRNAs, yet their functions and evolutionary origins remain largely unknown. The 24-nt meiotic phasiRNAs have only been described in grasses, wherein their biogenesis is dependent on a specialized Dicer (DCL5). To assess how evolution gave rise to this pathway, we examined reproductive phasiRNA pathways in nongrass monocots: garden asparagus, daylily, and lily. The common ancestors of these species diverged approximately 115-117 million years ago (MYA). We found that premeiotic 21-nt and meiotic 24-nt phasiRNAs were abundant in all three species and displayed spatial localization and temporal dynamics similar to grasses. The miR2275-triggered pathway was also present, yielding 24-nt reproductive phasiRNAs, and thus originated more than 117 MYA. In asparagus, unlike in grasses, these siRNAs are largely derived from inverted repeats (IRs); analyses in lily identified thousands of precursor loci, and many were also predicted to form foldback substrates for Dicer processing. Additionally, reproductive phasiRNAs were...Continue Reading

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Citations

Apr 18, 2019·The New Phytologist·Wenye Wu, Binglian Zheng
Jan 4, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yue LuYu Zhao
Aug 14, 2019·Annual Review of Cell and Developmental Biology·Yu YuYueqin Chen
Feb 6, 2020·Frontiers in Plant Science·Xiaoning Lei, Bing Liu
Mar 7, 2020·BMC Genomics·Rongzhi ZhangGenying Li
Oct 17, 2020·Nature Communications·Pengfei JiangYijun Qi
Dec 18, 2020·Briefings in Bioinformatics·A T Vivek, Shailesh Kumar
Feb 6, 2021·RNA Biology·Yuhan FeiJi Huang
Dec 28, 2019·Current Opinion in Plant Biology·Hiu Tung ChowRebecca A Mosher
May 11, 2021·Frontiers in Molecular Biosciences·Jiali YuMargaret E Staton
Jun 24, 2021·The Plant Journal : for Cell and Molecular Biology·Suresh PokhrelBlake C Meyers

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