DOI: 10.1101/485581Dec 4, 2018Paper

Plant-derived coumarins shape the composition of an Arabidopsis synthetic root microbiome

BioRxiv : the Preprint Server for Biology
Mathias J.E.E.E. VogesElizabeth S Sattely

Abstract

Which factors dictate the composition of the root microbiome and its role in plant fitness is a long-standing question. Recent work has highlighted a major contribution of the soil inoculum in determining the composition of the root microbiome. However, plants are known to conditionally exude a diverse array of unique secondary metabolites, largely varying between species and environmental conditions. Here, we explore the role of specialized metabolites in dictating which bacteria reside in the rhizosphere. We employed a reduced synthetic community (SynCom) of Arabidopsis thaliana root-isolated bacteria to detect community shifts that occur in the absence of the secreted small molecule phytoalexins, flavonoids, and coumarins. We find that lack of coumarin biosynthesis in f6'h1 mutant plant lines causes a shift in the root microbial community specifically under iron deficiency. We demonstrate a potential role for iron-mobilizing coumarins in sculpting the A. thaliana root bacterial community by inhibiting the proliferation of a relatively abundant Pseudomonas species via a redox-mediated mechanism. This work establishes a systematic approach enabling elucidation of specific mechanisms by which plant-derived molecules mediate mic...Continue Reading

Related Concepts

Coumarin
Environment
Oxidation-Reduction
Arabidopsis thaliana <plant>
Base
Iron Deficiency
Plant Roots
Shapes
Cell Proliferation
Flavonoids

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