Plant fatty acid (ethanol) amide hydrolases

Biochimica Et Biophysica Acta
Rhidaya ShresthaKent D Chapman

Abstract

Fatty acid amide hydrolase (FAAH) plays a central role in modulating endogenous N-acylethanolamine (NAE) levels in vertebrates, and, in part, constitutes an "endocannabinoid" signaling pathway that regulates diverse physiological and behavioral processes in animals. Recently, an Arabidopsis FAAH homologue was identified which catalyzed the hydrolysis of NAEs in vitro suggesting a FAAH-mediated pathway exists in plants for the metabolism of endogenous NAEs. Here, we provide evidence to support this concept by identifying candidate FAAH genes in monocots (Oryza sativa) and legumes (Medicago truncatula), which have similar, but not identical, exon-intron organizations. Corresponding M. truncatula and rice cDNAs were isolated and cloned into prokaryotic expression vectors and expressed as recombinant proteins in Escherichia coli. NAE amidohydrolase assays confirmed that these proteins indeed catalyzed the hydrolysis of 14C-labeled NAEs in vitro. Kinetic parameters and inhibition properties of the rice FAAH were similar to those of Arabidopsis and rat FAAH, but not identical. Sequence alignments and motif analysis of plant FAAH enzymes revealed a conserved domain organization for these members of the amidase superfamily. Five amino-...Continue Reading

Citations

Aug 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuh-Shuh WangElison B Blancaflor
Jan 31, 2012·Plant Science : an International Journal of Experimental Plant Biology·Denis CoulonJean-Jacques Bessoule
Apr 21, 2009·Medicinal Research Reviews·Raju Gautam, Sanjay M Jachak
Jul 2, 2010·British Journal of Pharmacology·Jürg GertschVincenzo Di Marzo
Jan 9, 2014·The Plant Journal : for Cell and Molecular Biology·Elison B BlancaflorKent D Chapman
Mar 5, 2011·The Journal of Biological Chemistry·Aruna KilaruKent D Chapman
Aug 23, 2007·Chemistry & Biodiversity·Aruna KilaruKent D Chapman
Feb 22, 2014·The Journal of Biological Chemistry·Lionel FaureKent D Chapman

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