Plant γH2AX foci are required for proper DNA DSB repair responses and colocalize with E2F factors.

The New Phytologist
Julien LangMarie-Edith Chabouté

Abstract

Cellular responses to DNA double-strand breaks (DSBs) are linked in mammals and yeasts to the phosphorylated histones H2AX (γH2AX) repair foci which are multiproteic nuclear complexes responsible for DSB sensing and signalling. However, neither the components of these foci nor their role are yet known in plants. In this paper, we describe the effects of γH2AX deficiency in Arabidopsis thaliana plants challenged with DSBs in terms of genotoxic sensitivity and E2F-mediated transcriptional responses. We further establish the existence, restrictive to the G1/S transition, of specific DSB-induced foci containing tobacco E2F transcription factors, in both A. thaliana roots and BY-2 tobacco cells. These E2F foci partially colocalize with γH2AX foci while their formation is ataxia telangiectasia mutated (ATM)-dependent, requires the E2F transactivation domain with its retinoblastoma-binding site and is optimal in the presence of functional H2AXs. Overall, our results unveil a new interplay between plant H2AX and E2F transcriptional activators during the DSB response.

References

Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Sep 9, 1999·The Journal of Cell Biology·E P RogakouW M Bonner
Nov 26, 1999·FEBS Letters·M SekineA Shinmyo
Sep 26, 2001·The Journal of Biological Chemistry·S BurmaD J Chen
Oct 24, 2001·The Journal of Biological Chemistry·I M Ward, J Chen
Mar 9, 2002·The Journal of Biological Chemistry·Marie-Edith ChaboutéGabriel Philipps
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Craig H BassingFrederick W Alt
Nov 7, 2002·Current Biology : CB·Junya KobayashiKenshi Komatsu
Jan 2, 2003·The Plant Cell·Valérie GarciaAlain Tissier
Feb 28, 2003·Nature·Grant S StewartStephen J Elledge
Apr 17, 2003·Molecular and Cellular Biology·Kang LiuWeei-Chin Lin
Jun 7, 2003·Nature Cell Biology·Arkady CelesteAndré Nussenzweig
Aug 14, 2003·Cell·Arkady CelesteAndré Nussenzweig
Sep 13, 2003·The Plant Journal : for Cell and Molecular Biology·I-Peng ChenHolger Puchta
Apr 13, 2004·The Plant Cell·Kevin CulliganAnne Britt
Jul 29, 2004·DNA Repair·Craig H Bassing, Frederick W Alt
Jul 29, 2004·DNA Repair·Craig Stevens, Nicholas B La Thangue
Nov 30, 2005·Mutagenesis·Hiroyuki Niida, Makoto Nakanishi
Aug 16, 2006·Oncogene·L-J KongJ R Nevins
Jan 18, 2007·The Plant Journal : for Cell and Molecular Biology·Kevin M CulliganAnne B Britt
May 10, 2007·PloS One·Lilian RicaudMarie-Hélène Montané
Aug 4, 2007·The Plant Journal : for Cell and Molecular Biology·Wanda M WaterworthChristopher E West
Aug 22, 2007·Molecular and Cellular Biology·Tsuyoshi IkuraKenji Kamiya
Nov 17, 2007·Science·Nadine K KolasDaniel Durocher
Apr 24, 2008·Cell Division·Joseph Ha VissersElisabetta Citterio
Sep 6, 2008·Nucleic Acids Research·Andrea KinnerGeorge Iliakis
Jan 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·David ShechterC David Allis
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kaoru YoshiyamaAnne B Britt
Jul 3, 2009·Plant Physiology·Hélène RoaMarie-Edith Chabouté
Dec 17, 2009·Sub-cellular Biochemistry·David Miguel Susano Pinto, Andrew Flaus

❮ Previous
Next ❯

Citations

Aug 14, 2012·Journal of Agricultural and Food Chemistry·Tong WuShuzhen Zhang
Apr 20, 2016·Journal of Environmental Sciences (China)·Tong WuShuzhen Zhang
Jun 3, 2015·Journal of Experimental Botany·Bingwei ZhangHengxiu Yu
Sep 11, 2012·Current Opinion in Plant Biology·Ruben Gutzat, Ortrun Mittelsten Scheid
Dec 3, 2014·Biochemical and Biophysical Research Communications·Shumin ZhouWei Zhang
May 20, 2015·Trends in Plant Science·Tomokazu KawashimaFrederic Berger
Dec 8, 2016·Nucleic Acids Research·Katiuska González-ArzolaIrene Díaz-Moreno
Feb 6, 2017·The Plant Journal : for Cell and Molecular Biology·Takeshi HirakawaSachihiro Matsunaga
Dec 17, 2015·Molecular and Cellular Biology·Ilaria Rebay
Jan 31, 2019·Journal of Integrative Plant Biology·En XieZhukuan Cheng
Feb 23, 2020·Journal of Experimental Botany·Isabel Bäurle, Inês Trindade
Mar 18, 2015·The Plant Journal : for Cell and Molecular Biology·Wangbin ZhouWen-Hui Shen
Sep 1, 2020·The EMBO Journal·Bénédicte Desvoyes, Crisanto Gutierrez
Feb 21, 2018·PLoS Genetics·Eleazar RodriguezMorten Petersen
Aug 17, 2019·Frontiers in Plant Science·Takeshi Hirakawa, Sachihiro Matsunaga
Nov 9, 2019·Trends in Plant Science·Laurent CambordeElodie Gaulin
Nov 7, 2021·The Plant Journal : for Cell and Molecular Biology·José Antonio Pedroza-GarciaLieven De Veylder

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.

Ataxia telangiectasia

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.