Plant organellar DNA polymerases paralogs exhibit dissimilar nucleotide incorporation fidelity

The FEBS Journal
Víctor M Ayala-GarcíaLuis G Brieba

Abstract

The coding sequences of plant mitochondrial and chloroplast genomes present a lower mutation rate than the coding sequences of animal mitochondria. However, plant mitochondrial genomes frequently rearrange and present high mutation rates in their noncoding sequences. DNA replication in plant organelles is carried out by two DNA polymerases (DNAP) paralogs. In Arabidopsis thaliana at least one DNAP paralog (AtPolIA or AtPolIB) is necessary for plant viability, suggesting that both genes are partially redundant. To understand how AtPolIs replicate genomes that present low and high mutation rates, we measured their nucleotide incorporation for all 16-base pair combinations in vitro. AtPolIA presents an error rate of 7.26 × 10-5 , whereas AtPolIB has an error rate of 5.45 × 10-4 . Thus, AtPolIA and AtPolIB are 3.5 and 26-times less accurate than human mitochondrial DNAP γ. The 8-fold difference in fidelity between both AtPolIs results from a higher catalytic efficiency in AtPolIA. Both AtPolIs extend from mismatches and the fidelity of AtPolIs ranks between high fidelity and lesion bypass DNAPs. The different nucleotide incorporation fidelity between AtPolIs predicts a prevalent role of AtPolIA in DNA replication and AtPolIB in DNA...Continue Reading

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Citations

Jan 31, 2019·Nucleic Acids Research·Paola L García-MedelLuis G Brieba
Feb 29, 2020·The Biochemical Journal·Noe Baruch-TorresLuis G Brieba
Oct 2, 2019·International Journal of Molecular Sciences·Jose-Antonio Pedroza-GarciaCécile Raynaud
Sep 25, 2019·Plants·Stewart A MorleyBrent L Nielsen
Jul 1, 2020·Proceedings of the National Academy of Sciences of the United States of America·Zhiqiang WuDaniel B Sloan

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