Plant-produced recombinant influenza vaccine based on virus-like HBc particles carrying an extracellular domain of M2 protein

Biochemistry. Biokhimii︠a︡
N V RavinK G Skryabin

Abstract

Conventional influenza vaccines are based on a virus obtained in chicken embryos or its components. The high variability of the surface proteins of influenza virus, hemagglutinin and neuraminidase, requires strain-specific vaccines matching the antigenic specificity of newly emerging virus strains to be developed. A recombinant vaccine based on a highly conservative influenza virus protein M2 fused to a nanosized carrier particle can be an attractive alternative to traditional vaccines. We have constructed a recombinant viral vector based on potato X virus that provides for expression in the Nicotiana benthamiana plants of a hybrid protein M2eHBc consisting of an extracellular domain of influenza virus M2 protein (M2e) fused to hepatitis B core antigen (HBc). This vector was introduced into plant cells by infiltrating leaves with agrobacteria carrying the viral vector. The hybrid protein M2eHBc was synthesized in the infected N. benthamiana plants in an amount reaching 1-2% of the total soluble protein and formed virus-like particles with the M2e peptide presented on the surface. Methods of isolation and purification of M2eHBc particles from plant producers were elaborated. Experiments on mice have shown a high immunogenicity o...Continue Reading

References

Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·Olivier VoinnetDavid Baulcombe
Mar 5, 2004·Plant Cell Reports·M E HornJ A Howard
May 28, 2004·Virus Research·W FiersW Min Jou
May 10, 2005·Immunology and Cell Biology·M Carmen CañizaresGeorge P Lomonossoff
May 26, 2005·Virology·Marina De FiletteWalter Fiers
Aug 23, 2005·Journal of Virological Methods·I A MechtcheriakovaG P Lomonossoff
Nov 30, 2005·Journal of Pharmaceutical Sciences·Roxana M IonescuLi Shi
Sep 19, 2006·Biochemistry. Biokhimii︠a︡·T V KomarovaJ G Atabekov
Mar 21, 2007·Current Opinion in Biotechnology·Yuri GlebaSylvestre Marillonnet
Jul 24, 2007·Human Vaccines·Jessica A Chichester, Vidadi Yusibov
Dec 8, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nicholas A CellarScott A Young
Jan 1, 2008·Influenza and Other Respiratory Viruses·Vadim MettVidadi Yusibov
Oct 21, 2009·Vaccine·Walter FiersXavier Saelens

❮ Previous
Next ❯

Citations

Jun 28, 2014·BioMed Research International·Hyun-Soon KimKisung Ko
Mar 6, 2015·Molecular Biotechnology·Aleksey FirsovSergey Dolgov
Sep 22, 2012·Human Vaccines & Immunotherapeutics·Qiang Chen, Huafang Lai
Jan 7, 2015·Plant Molecular Biology·Jorge A Salazar-GonzálezSergio Rosales-Mendoza
May 21, 2013·Trends in Microbiology·Kanta Subbarao, Yumiko Matsuoka
Oct 19, 2016·International Journal of Molecular Sciences·Young Hee JoungHyun-Soon Kim
Dec 17, 2015·Therapeutic Advances in Vaccines·Natsumi TakeyamaYoshikazu Yuki
Sep 6, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Edward P Rybicki
Feb 19, 2013·Expert Review of Vaccines·Nunzia Scotti, Edward P Rybicki
Nov 25, 2020·Vaccines·Nathaniel Nyakaat NinyioAbdul Razak Mariatulqabtiah
Mar 19, 2021·Biotechnology Reports·Maryam Moradi VahdatAli Niazi
Nov 28, 2021·Biotechnology Letters·Farshad HemmatiAlireza Afsharifar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.