Plant regeneration and stable transformation in the floricultural plant Cleome spinosa, a C₃ plant closely related to the C₄ plant C. gynandra.

Plant Cell Reports
Yung-Ting TsaiKin-Ying To

Abstract

Cleome spinosa is widely used as a garden ornamental in many countries. Here we determined the optimal conditions for plant regeneration from different tissue explants grown in vitro. Induction medium containing MS salts, MS vitamins, 3% sucrose, 1 mg l⁻¹ BA, 200 mg l⁻¹ timentin, and 0.8% agar was sufficient for shoot regeneration of all the tissue explants examined, including leaf, hypocotyl, and cotyledon. Subsequently, an Agrobacterium tumefaciens-mediated method was developed to transform the vector pCHS, which carries the transgenes Petunia chalcone synthase (chs) and selection marker neomycin phosphotransferase II (nptII), into C. spinosa. From a total of 368 cotyledon explants, 13 putative transgenic lines were regenerated from selection medium supplemented with 50 mg l⁻¹ kanamycin and 200 mg l⁻¹ timentin, and transferred to the greenhouse. Genomic PCR and Southern blot analyses revealed that the nptII transgene was present in all 13 transgenic plants. Similarly, when the Petunia chs transgene was used as a probe in Southern blot analysis, single or multiple hybridization bands were detected in 12 out of the 13 transgenic plants. In addition, T₁ progeny assay from selected transformants showed that the nptII transgene ca...Continue Reading

References

Apr 5, 2001·Current Opinion in Biotechnology·G Forkmann, S Martens
Dec 21, 2002·Journal of Experimental Botany·E RavehE E Goldschmidt
Sep 19, 2003·Plant Molecular Biology·Sung-Ryul KimGynheung An
Feb 28, 2004·Journal of Natural Products·Dwight O CollinsPaul B Reese
Sep 13, 2011·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·T T H DaoR Verpoorte
May 1, 2007·Functional Plant Biology : FPB·Elena V VoznesenskayaGerald E Edwards

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Citations

Jun 25, 2013·Photosynthesis Research·Sarah CovshoffBritta M C Kümpers
Jun 19, 2021·Applications in Plant Sciences·Shane CareyJocelyn C Hall

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